A monoclonal antibody collection for C. difficile typing ?

Autor: Hunault L; Antibodies in Therapy and Pathology, Institut Pasteur, Université Paris Cité, INSERM UMR1222, 75015, Paris, France.; Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Sorbonne Université, INSERM, CNRS, 75013, Paris, France.; Sorbonne Université, Collège doctoral, 75005, Paris, France., England P; Plateforme de Biophysique Moléculaire, Institut Pasteur, Université Paris Cité, CNRS UMR3528, 75015, Paris, France., Barbut F; National Reference Laboratory for Clostridium difficile, 75012, Paris, France.; Université Paris Cité, INSERM UMR-1139, Paris, France., Iannascoli B; Antibodies in Therapy and Pathology, Institut Pasteur, Université Paris Cité, INSERM UMR1222, 75015, Paris, France., Godon O; Antibodies in Therapy and Pathology, Institut Pasteur, Université Paris Cité, INSERM UMR1222, 75015, Paris, France., Déjardin F; Production and Purification of Recombinant Proteins Facility, Institut Pasteur, 75015, Paris, France., Thomas C; Production and Purification of Recombinant Proteins Facility, Institut Pasteur, 75015, Paris, France., Dupuy B; UMR-CNRS 6047, Laboratoire Pathogenèse des Bactéries Anaérobies, Institut Pasteur, Université Paris-Cité, 75015, Paris, France., Guo C; Regneron Pharmaceuticals, Tarrytown, NY, USA., Macdonald L; Regneron Pharmaceuticals, Tarrytown, NY, USA., Gorochov G; Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Sorbonne Université, INSERM, CNRS, 75013, Paris, France. guy.gorochov@sorbonne-universite.fr., Sterlin D; Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Sorbonne Université, INSERM, CNRS, 75013, Paris, France., Bruhns P; Antibodies in Therapy and Pathology, Institut Pasteur, Université Paris Cité, INSERM UMR1222, 75015, Paris, France. pierre.bruhns@pasteur.fr.
Jazyk: angličtina
Zdroj: Gut pathogens [Gut Pathog] 2024 Jan 19; Vol. 16 (1), pp. 4. Date of Electronic Publication: 2024 Jan 19.
DOI: 10.1186/s13099-023-00592-7
Abstrakt: Clostridioides difficile is the leading cause of antibiotic-associated diarrhea and pseudomembranous colitis in adults. Various C. difficile strains circulate currently, associated with different outcomes and antibiotic resistance profiles. However, most studies still focus on the reference strain 630 that does not circulate anymore, partly due to the lack of immunological tools to study current clinically important C. difficile PCR ribotypes. The goal of this study was to generate monoclonal antibodies recognizing various epidemic ribotypes of C. difficile. To do so, we immunized mice expressing human variable antibody genes with the Low Molecular Weight (LMW) subunit of the surface layer protein SlpA from various C. difficile strains. Monoclonal antibodies purified from hybridomas bound LMW with high-affinity and whole bacteria from current C. difficile ribotypes with different cross-specificities. This first collection of anti-C. difficile mAbs represent valuable tools for basic and clinical research.
(© 2024. The Author(s).)
Databáze: MEDLINE
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