Adiponectin Receptor Agonist Effectively Suppresses Hepatocellular Carcinoma Growth.

Autor: Nguyen MLT; Vietnam Military Medical University, Hanoi, Vietnam.; Department of Biochemistry, Military Hospital 103, Hanoi, Vietnam., Pham C; Laboratory Animal Research Centre, Vietnam Military Medical University, Hanoi, Vietnam., Pham VT; Vietnam Military Medical University, Hanoi, Vietnam.; Department of Biochemistry, Military Hospital 103, Hanoi, Vietnam., Nham PLT; Laboratory Animal Research Centre, Vietnam Military Medical University, Hanoi, Vietnam., Ta BT; Vietnam Military Medical University, Hanoi, Vietnam.; Respiratory Centre, Military Hospital 103, Hanoi, Vietnam., Le DT; Vietnam Military Medical University, Hanoi, Vietnam.; Department of Rheumatology and Endocrinology, Military Hospital 103, Hanoi, Vietnam., Le QV; Vietnam Military Medical University, Hanoi, Vietnam.; Department of Medical Examination, Le Huu Trac National Burn Hospital, Hanoi, Vietnam., Hoang XC; Vietnam Military Medical University, Hanoi, Vietnam., Bozko P; Department of Internal medicine I, Universitätsklinikum Tübingen, Tübingen, Germany.; The M3 Research Institute, Tübingen, Germany., Nguyen LT; Vietnam Military Medical University, Hanoi, Vietnam.; Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam.; Vietnamese-German Center for Medical Research (VG-CARE), Hanoi, Vietnam., Bui KC; Vietnam Military Medical University, Hanoi, Vietnam. buikhaccuong@gmail.com.; Laboratory Animal Research Centre, Vietnam Military Medical University, Hanoi, Vietnam. buikhaccuong@gmail.com.; Department of Internal medicine I, Universitätsklinikum Tübingen, Tübingen, Germany. buikhaccuong@gmail.com.; Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam. buikhaccuong@gmail.com.; Vietnamese-German Center for Medical Research (VG-CARE), Hanoi, Vietnam. buikhaccuong@gmail.com.
Jazyk: angličtina
Zdroj: Cell biochemistry and biophysics [Cell Biochem Biophys] 2024 Jun; Vol. 82 (2), pp. 687-695. Date of Electronic Publication: 2024 Jan 20.
DOI: 10.1007/s12013-024-01217-9
Abstrakt: Hepatocellular carcinoma (HCC) is the second lethal cancer. Short overall survival, low five-year survival rate, and unimproved treatment efficacy urge the need to improve HCC prognosis. Adiponectin is key protector against cancer and hepatic abnormalities. Hypoadiponectinemia occurs in and promotes carcinogenesis and hepatic diseases. Adiponectin reactivation by different methods showed impressive effect against cancer and hepatic diseases. Recently, AdipoRon, an adiponectin receptor agonist, can interact with both Adiponectin receptors. AdipoRon showed promising anti-cancer effect in some cancers, but no study on HCC yet. The in vitro effect of AdipoRon on HCC was investigated by cell viability, migration, invasion, colony formation and apoptosis assays. The signalling alteration was determined by RT-qPCR and Western blot. The effect of treatment was interpreted by comparison between treatments and control. The difference between two cell lines was relatively compared. Our results showed significant in vitro anti-cancer effect of AdipoRon via AMPK- and dose-dependent manner. Huh7 cells showed a lower level of AdipoR1/2 and a superior proliferation and aggressiveness, compared to Hep3B. In addition, Huh7 cells were more sensitive to AdipoRon treatment (lower IC 50 , less cell growth, migration, invasion and colonies upon AdipoRon treatment) than Hep3B cells. In conclusion, AdipoRon effectively inhibited HCC growth and invasiveness in vitro. The deficient expression of adiponectin receptors affects efficacy of AdipoRon and aggressiveness of HCC cells.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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