Larvicidal potential, antimicrobial properties and molecular docking analysis of Egyptian Mint (Mentha rotundifolia) against Culex pipiens L. (Diptera: Culicidae) and Midgut-borne Staphylococcus aureus.

Autor: Abu-Hussien SH; Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams University, Cairo, 12411, Egypt. samah_hashem1@agr.asu.edu.eg., Hemdan B; Water Pollution Research Department, Environmental Research and Climate Change Institute, National Research Centre, 33 El-Bohouth St., Dokki, Giza, 12622, Egypt., Abd-Elhalim BT; Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams University, Cairo, 12411, Egypt., Aboul Fotouh MM; Department of Agriculture Biochemistry, Faculty of Agriculture, Ain Shams University, Cairo, 12411, Egypt., Soliman AG; Biotechnology program, New Programs, Faculty of Agriculture, Ain Shams University, Cairo, 12411, Egypt., Ghallab YK; Biotechnology program, New Programs, Faculty of Agriculture, Ain Shams University, Cairo, 12411, Egypt., Adly E; Department of Entomology, Faculty of Science, Ain Shams University, Cairo, 11566, Egypt. eslam.adly@sci.asu.edu.eg., El-Sayed SM; Department of Agriculture Biochemistry, Faculty of Agriculture, Ain Shams University, Cairo, 12411, Egypt.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jan 19; Vol. 14 (1), pp. 1697. Date of Electronic Publication: 2024 Jan 19.
DOI: 10.1038/s41598-024-51634-2
Abstrakt: Mosquitoes prefer stagnant areas near hospitals to live and easily spread pathogenic bacteria. Our current study aims to isolate multidrug-resistant (MDR) Staphylococcus aureus isolates from midguts of Mosquito Culex pipiens and study the potential of mint as a biocontrol strategy against C. pipiens larvae and their midgut-borne S. aureus. Samples of the third and fourth larval instars of C. pipiens were collected from water ponds around three Cairo hospitals. Ciprofloxacin, gentamycin and tetracycline, as well as various concentrations of mint leaf extract (MLE) were tested for antibiotic susceptibility. Sixty-five isolates were obtained and showed antibiotic resistance to tetracycline, gentamycin, ciprofloxacin, and undiluted MLE with resistant percentages (%) of 27.69, 30.76, 17.46, and 23.08%, respectively. Undiluted MLE inhibited 61.53% of the multidrug S. aureus isolates, whereas it couldn't inhibit any of these isolates at dilutions less than 50 μg/mL. The MIC of MLE was ≤ 700 µg/mL, while the MIC of the antibiotics ranged from 0.25 to 5.0 µg/mL for the three antibiotics. The most inhibited S. aureus isolate was identified by 16SrRNA sequencing approach and registered in GenBank as S. aureus MICBURN with gene accession number OQ766965. MLE killed all larval stages after 72 h of exposure, with mortality (%) reaching 93.33 and 100% causing external hair loss, breakage of the outer cuticle epithelial layer of the abdomen, and larvae shrinkage. Histopathology of treated larvae showed destruction of all midgut cells and organelles. Gas chromatography (GC) of MLE revealed that menthol extract (35.92%) was the largest active ingredient, followed by menthone (19.85%), D-Carvone (15.46%), Pulegone (5.0579%). Docking analysis confirmed that alpha guanine and cadinol had the highest binding affinity to both predicted active sites of Culex pipiens acetylcholinesterase. As a result, alpha-guanine and cadinol might have a role as acetylcholinesterase inhibitors.
(© 2024. The Author(s).)
Databáze: MEDLINE
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