Genome-wide association analysis identifies a consistent QTL for powdery mildew resistance on chromosome 3A in Nordic and Baltic spring wheat.

Autor: Lin M; Department of Plant Sciences, Norwegian University of Life Sciences, Post Box 5003, NO-1432, ÅS, Norway., Islamov B; Centre of Estonian Rural Research and Knowledge, J. Aamisepa 1, Jõgeva Alevik, 48309, Jõgeva Maakond, Estonia., Aleliūnas A; Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, 58344, Akademija, Lithuania., Armonienė R; Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, 58344, Akademija, Lithuania., Gorash A; Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, 58344, Akademija, Lithuania., Meigas E; Institute of Bioengineering, University of Tartu, Nooruse 1, 50411, Tartu, Estonia., Ingver A; Centre of Estonian Rural Research and Knowledge, J. Aamisepa 1, Jõgeva Alevik, 48309, Jõgeva Maakond, Estonia., Tamm I; Centre of Estonian Rural Research and Knowledge, J. Aamisepa 1, Jõgeva Alevik, 48309, Jõgeva Maakond, Estonia., Kollist H; Institute of Bioengineering, University of Tartu, Nooruse 1, 50411, Tartu, Estonia., Strazdiņa V; Institute of Agricultural Resources and Economics, Zinatnes Iela 2, Cesis County, 4126, Latvia., Bleidere M; Institute of Agricultural Resources and Economics, Zinatnes Iela 2, Cesis County, 4126, Latvia., Brazauskas G; Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, 58344, Akademija, Lithuania., Lillemo M; Department of Plant Sciences, Norwegian University of Life Sciences, Post Box 5003, NO-1432, ÅS, Norway. morten.lillemo@nmbu.no.
Jazyk: angličtina
Zdroj: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2024 Jan 19; Vol. 137 (1), pp. 25. Date of Electronic Publication: 2024 Jan 19.
DOI: 10.1007/s00122-023-04529-1
Abstrakt: Key Message: QPm.NOBAL-3A is an important QTL providing robust adult plant powdery mildew resistance in Nordic and Baltic spring wheat, aiding sustainable crop protection and breeding. Powdery mildew, caused by the biotrophic fungal pathogen Blumeria graminis f. sp. tritici, poses a significant threat to bread wheat (Triticum aestivum L.), one of the world's most crucial cereal crops. Enhancing cultivar resistance against this devastating disease requires a comprehensive understanding of the genetic basis of powdery mildew resistance. In this study, we performed a genome-wide association study (GWAS) using extensive field trial data from multiple environments across Estonia, Latvia, Lithuania, and Norway. The study involved a diverse panel of recent wheat cultivars and breeding lines sourced from the Baltic region and Norway. We identified a major quantitative trait locus (QTL) on chromosome 3A, designated as QPm.NOBAL-3A, which consistently conferred high resistance to powdery mildew across various environments and countries. Furthermore, the consistency of the QTL haplotype effect was validated using an independent Norwegian spring wheat panel. Subsequent greenhouse seedling inoculations with 15 representative powdery mildew isolates on a subset of the GWAS panel indicated that this QTL provides adult plant resistance and is likely of race non-specific nature. Moreover, we developed and validated KASP markers for QPm.NOBAL-3A tailored for use in breeding. These findings provide a critical foundation for marker-assisted selection in breeding programs aimed at pyramiding resistance QTL/genes to achieve durable and broad-spectrum resistance against powdery mildew.
(© 2024. The Author(s).)
Databáze: MEDLINE
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