Erythritol alters phosphotransferase gene expression and inhibits the in vitro growth of Staphylococcus coagulans isolated from canines with pyoderma.

Autor:	Onishi-Sakamoto S; Cooperative Division of Veterinary Sciences, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan., Fujii T; Department of Gastroenterology and Hepatology, Fujita Health University, Toyoake, Japan.; Department of Medical Research on Prebiotics and Probiotics, Fujita Health University, Toyoake, Japan., Watanabe K; Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan., Makida R; Research and Development Center, B Food Science Co., Ltd., Chita, Japan., Iyori K; Dermatological and Laboratory Service for Animals, Vet Derm Tokyo, Fujisawa, Japan., Toyoda Y; Dermatological and Laboratory Service for Animals, Vet Derm Tokyo, Fujisawa, Japan., Tochio T; Department of Gastroenterology and Hepatology, Fujita Health University, Toyoake, Japan.; Department of Medical Research on Prebiotics and Probiotics, Fujita Health University, Toyoake, Japan., Nishifuji K; Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2024 Jan 04; Vol. 10, pp. 1272595. Date of Electronic Publication: 2024 Jan 04 (Print Publication: 2023).
DOI:	10.3389/fvets.2023.1272595
Abstrakt:	Staphylococcus coagulans (SC) belongs to a group of coagulase-positive staphylococci occasionally isolated from the skin lesions of dogs with pyoderma. We recently revealed that erythritol, a sugar alcohol, inhibited the growth of SC strain JCM7470. This study investigated the molecular mechanisms involved in this growth inhibition of JCM7470 by erythritol, and determine whether erythritol inhibits the growth of SC isolated from the skin of dogs with pyoderma. Comprehensive analysis of the gene expression of JCM7470 in the presence of erythritol revealed that erythritol upregulated the expression of glcB and ptsG genes, both of which encode phosphotransferase system (PTS) glucoside- and glucose-specific permease C, B, and A domains (EIICBA), respectively, associated with sugar uptake. Moreover, erythritol suppressed in vitro growth of all 27 SC strains isolated from the skin lesions of canine pyoderma, including 13 mecA gene-positive and 14 mecA gene-negative strains. Finally, the growth inhibition of the SC clinical isolates by erythritol was restored by the addition of glucose. In summary, we revealed that erythritol promotes PTS gene expression and suppresses the in vitro growth of SC clinical isolates from dogs with pyoderma. Restoration of the erythritol-induced growth inhibition by glucose suggested that glucose starvation may contribute to the growth inhibition of SC. Competing Interests: RM is an employee of B Food Science Co., Ltd. TF and TT were employees of the same company at the time when this study was started. KI was employed by Vet Derm Tokyo Ltd. where YT the representative director. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Onishi-Sakamoto, Fujii, Watanabe, Makida, Iyori, Toyoda, Tochio and Nishifuji.)
Databáze:	MEDLINE
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