Dynamics of HLA and angiotensin II type 1 receptor antibodies during pregnancy.

Autor: Burballa C; Department of Nephrology, Hospital del Mar, Barcelona, Spain; Nephropathies Research Group, Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain., Llinàs-Mallol L; Department of Nephrology, Hospital del Mar, Barcelona, Spain; Nephropathies Research Group, Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain., Vázquez S; Department of Nephrology, Hospital del Mar, Barcelona, Spain; Nephropathies Research Group, Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain., Pérez-Sáez MJ; Department of Nephrology, Hospital del Mar, Barcelona, Spain; Nephropathies Research Group, Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain., Arias-Cabrales C; Department of Nephrology, Hospital del Mar, Barcelona, Spain; Nephropathies Research Group, Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain., Buxeda A; Department of Nephrology, Hospital del Mar, Barcelona, Spain; Nephropathies Research Group, Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain., Hernandez JL; Department of Obstetrics and Gynecology, Hospital del Mar, Barcelona, Spain., Riera M; Nephropathies Research Group, Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain., Sanz S; Nephropathies Research Group, Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain., Alari-Pahissa E; Nephropathies Research Group, Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain., Federico-Vega J; Nephropathies Research Group, Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain., Eguía J; Laboratori de Referència de Catalunya SA, Barcelona, Spain., Pascual J; Nephropathies Research Group, Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain; Department of Nephrology, Hospital 12 de Octubre, Madrid, Spain., Redondo-Pachón D; Department of Nephrology, Hospital del Mar, Barcelona, Spain; Nephropathies Research Group, Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain., Crespo M; Department of Nephrology, Hospital del Mar, Barcelona, Spain; Nephropathies Research Group, Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain. Electronic address: mcrespo@psmar.cat.
Jazyk: angličtina
Zdroj: Human immunology [Hum Immunol] 2024 Mar; Vol. 85 (2), pp. 110749. Date of Electronic Publication: 2024 Jan 18.
DOI: 10.1016/j.humimm.2024.110749
Abstrakt: Background: Alloantibodies, especially anti-human leukocyte antigen antibodies (HLA antibodies), and autoantibodies, as angiotensin II type 1 receptor antibodies (AT1R antibodies), may complicate the access and the course of transplantation. Pregnancy is a known source of HLA antibodies, with most studies evaluating pregnancy-induced sensitization by complement-dependent cytotoxicity assays, mainly after childbirth. AT1R antibodies have been evaluated in the context of preeclampsia. We aimed to evaluate pregnancy as a natural source of HLA antibodies and AT1R antibodies, their dynamics along gestation and the potential factors involved in antibody appearance.
Methods: Serum samples from pregnant women were collected during the three trimesters of pregnancy (1T, 2T, 3T). Presence of HLA antibodies was assessed by screening beads on Luminex and AT1R antibodies by ELISA.
Results: A cohort of 138 pregnant women were included. Samples from all were tested in 1T, 127 in 2T and 102 in 3T. HLA antibodies increased from 29.7 % (1T) to 38.2 % (3T). AT 1 R antibodies were stable around 30 % along pregnancy. Up to 43.2 % multiparous women had HLA antibodies, with a similar proportion of class I and class II antibodies. In primiparous women HLA antibodies increased along pregnancy (from 17.6 % to 34.1 %), with predominance of class II HLA antibodies. AT 1 R antibodies were not different in primiparous and multiparous women.
Conclusions: Pregnancy is a relevant source of HLA antibodies sensitization, but not of AT 1 R antibodies. HLA antibodies increased clearly in primiparous women with predominance of class II. The use of newer solid-phase techniques on Luminex evidence a higher degree of HLA sensitization during pregnancy.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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