Multilocus sequence typing schemes for the emerging swine pathogen Mycoplasma hyosynoviae.

Autor: Bünger M; University of Veterinary Medicine Vienna, University Clinic for Swine, Veterinärplatz 1, 1210 Vienna, Austria., Blümlinger M; University of Veterinary Medicine Vienna, University Clinic for Swine, Veterinärplatz 1, 1210 Vienna, Austria., Loncaric I; University of Veterinary Medicine Vienna, Institute of Microbiology, Veterinärplatz 1, 1210 Vienna, Austria., Rosel AC; Austrian Agency for Health and Food Safety, Institute of Medical Microbiology and Hygiene, Währinger Straße 25A, 1096 Vienna, Austria., Ruppitsch W; Austrian Agency for Health and Food Safety, Institute of Medical Microbiology and Hygiene, Währinger Straße 25A, 1096 Vienna, Austria., Teich K; SAN Group Biotech Germany GmbH (former ANICON Labor GmbH), Mühlenstraße 13, 49685 Höltinghausen, Germany., Kübber-Heiss A; University of Veterinary Medicine Vienna, Research Institute of Wildlife Ecology, Savoyenstraße 1, 1160 Vienna, Austria., Hennig-Pauka I; University of Veterinary Medicine Hannover, Foundation, Field Station for Epidemiology, Buescheler Straße 9, 49456 Bakum, Germany., Ladinig A; University of Veterinary Medicine Vienna, University Clinic for Swine, Veterinärplatz 1, 1210 Vienna, Austria., Spergser J; University of Veterinary Medicine Vienna, Institute of Microbiology, Veterinärplatz 1, 1210 Vienna, Austria. Electronic address: joachim.spergser@vetmeduni.ac.at.
Jazyk: angličtina
Zdroj: Veterinary microbiology [Vet Microbiol] 2024 Mar; Vol. 290, pp. 109997. Date of Electronic Publication: 2024 Jan 15.
DOI: 10.1016/j.vetmic.2024.109997
Abstrakt: Mycoplasma (M.) hyosynoviae is a commensal of the upper respiratory tract in swine, which has the potential to spread systemically, usually resulting in arthritis in fattening pigs and gilts. To date, very little is known about the epidemiology of M. hyosynoviae, mainly due to a lack of suitable typing methods. Therefore, this study aimed to develop both a conventional multi locus sequence typing (MLST) and a core genome (cg) MLST scheme. The development of the cgMLST was based on whole genome sequences of 64 strains isolated from pigs and wild boars during routine diagnostics as well as nine publicly available genomes. A cgMLST scheme containing 390 target genes was established using the Ridom© SeqSphere+ software. Using this scheme as a foundation, seven housekeeping genes were selected for conventional MLST based on their capability to reflect genome wide relatedness and subsequently, all 73 strains were typed by applying both methods. Core genome MLST results revealed a high diversity of the studied strain population and less than 100 allele differences between epidemiologically unrelated strains were only detected for four isolates from the US. On the other hand, seven clonal clusters (≤ 12 allele differences) comprising 20 isolates were identified. Comparison of the two typing methods resulted in highly congruent phylogenetic trees and an Adjusted Rand Coefficient of 0.893, while cgMLST showed marginally higher resolution when comparing closely related isolates, indicated by a slightly higher Simpson's ID (0.992) than conventional MLST (Simpson's ID = 0.990). Overall, both methods seem well suited for epidemiological analyses for scientific as well as diagnostic purposes. While MLST is faster and cheaper, cgMLST can be used to further differentiate closely related isolates.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: