| Autor: |
Chua GNL; Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University, New York, New York, USA; email: shixinliu@rockefeller.edu.; Tri-Institutional PhD Program in Chemical Biology, New York, New York, USA., Liu S; Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University, New York, New York, USA; email: shixinliu@rockefeller.edu. |
| Jazyk: |
angličtina |
| Zdroj: |
Annual review of biophysics [Annu Rev Biophys] 2024 Jul; Vol. 53 (1), pp. 169-191. Date of Electronic Publication: 2024 Jun 28. |
| DOI: |
10.1146/annurev-biophys-030822-032904 |
| Abstrakt: |
Myriad DNA-binding proteins undergo dynamic assembly, translocation, and conformational changes while on DNA or alter the physical configuration of the DNA substrate to control its metabolism. It is now possible to directly observe these activities-often central to the protein function-thanks to the advent of single-molecule fluorescence- and force-based techniques. In particular, the integration of fluorescence detection and force manipulation has unlocked multidimensional measurements of protein-DNA interactions and yielded unprecedented mechanistic insights into the biomolecular processes that orchestrate cellular life. In this review, we first introduce the different experimental geometries developed for single-molecule correlative force and fluorescence microscopy, with a focus on optical tweezers as the manipulation technique. We then describe the utility of these integrative platforms for imaging protein dynamics on DNA and chromatin, as well as their unique capabilities in generating complex DNA configurations and uncovering force-dependent protein behaviors. Finally, we give a perspective on the future directions of this emerging research field. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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