Identification of Increased Grain Length 1 (IGL1), a novel gene encoded by a major QTL for modulating grain length in rice.

Autor: Niu J; College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China., Wang F; College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China., Yang C; College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China., Ye Q; College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China., Huang J; College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China., La Y; College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China., Wang Q; College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China., Dai J; Academy for Advanced Interdisciplinary Studies, College of Engineering, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China., Hu T; College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China., Sang L; College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China., Zhang P; Anhui Province Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230041, Anhui, China., Zou Y; Anhui Province Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230041, Anhui, China., Zhai Z; College of Artificial Intelligence, Nanjing Agricultural University, Nanjing, 210095, China., Jin J; State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China., Abdulmajid D; Rice Research and Training Centre, Field Crops Research Institute, Agricultural Research Centre, Kafr El-Sheikh, 33717, Kafr El-Sheikh Governorate, Egypt., Guo J; Centre in Artificial Intelligence Driven Drug Discovery, Faculty of Applied Sciences, Macao Polytechnic University, Macao, 999078, China., Chen H; College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China. chenhuh@njau.edu.cn., La H; College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China. hongguila@njau.edu.cn.
Jazyk: angličtina
Zdroj: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2024 Jan 18; Vol. 137 (1), pp. 24. Date of Electronic Publication: 2024 Jan 18.
DOI: 10.1007/s00122-023-04531-7
Abstrakt: Key Message: A novel quantitative trait locus qIGL1, which performed a positive function in regulating grain length in rice, was cloned by the map-based cloning approach; further studies revealed that it corresponded to LOC_Os03g30530, and the IGL1 appeared to contribute to lengthening and widening of the cells on the surface of grain hulls. Grain length is a prominent determinant for grain weight and appearance quality of rice. In this study, we conducted quantitative trait locus mapping to determine a genomic interval responsible for a long-grain phenotype observed in a japonica cultivar HD385. This led to the identification of a novel QTL for grain length on chromosome 3, named qIGL1 (for Increased Grain Length 1); the HD385 (Handao 385)-derived allele showed enhancement effects on grain length, and such an allele as well as NIP (Nipponbare)-derived allele was designated qigl1 HD385 and qIGL1 NIP , respectively. Genetic analysis revealed that the qigl1 HD385 allele displayed semidominant effects on grain length. Fine mapping further narrowed down the qIGL1 to an ~ 70.8-kb region containing 9 open reading frames (ORFs). A comprehensive analysis indicated that LOC_Os03g30530, which corresponded to ORF6 and carried base substitutions and deletions in HD385 relative to NIP, thereby causing changes or losses of amino-acid residues, was the true gene for qIGL1. Comparison of grain traits between a pair of near-isogenic lines (NILs), termed NIL-igl1 HD385 and NIL-IGL1 NIP , discovered that introduction of the igl1 HD385 into the NIP background significantly resulted in the elevations of grain length and 1000-grain weight. Closer inspection of grain surfaces revealed that the cell length and width in the longitudinal direction were significantly longer and greater, respectively, in NIL-igl1 HD385 line compared with in NIL-IGL1 NIP line. Hence, our studies identified a new semidominant natural allele contributing to the increase of grain length and further shed light on the regulatory mechanisms of grain length.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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