A Mid-Density Single-Nucleotide Polymorphism Panel for Molecular Applications in Cowpea ( Vigna unguiculata (L.) Walp).

Autor: Ongom PO; International Institute of Tropical Agriculture (IITA), Kano, Nigeria., Fatokun C; International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria., Togola A; International Institute of Tropical Agriculture (IITA), Kano, Nigeria., Garcia-Oliveira AL; International Maize and Wheat Improvement Center (CIMMYT), ICRAF House, UN Avenue, PO Box, Nairobi 1041-00621, Kenya.; Department of Molecular Biology, College of Biotechnology, CCS Haryana Agricultural University, Hisar, India., Ng EH; Excellence in Breeding Platform, International Maize and Wheat Improvement Center (CIMMYT), Los Baños, Laguna 4031, Philippines., Kilian A; Diversity Arrays Technology Pty Ltd., University of Canberra, Montana St., Bruce, ACT 2617, Australia., Lonardi S; Department of Computer Science and Engineering, University of California, 900 University Avenue, Riverside, CA 92521, USA., Close TJ; Department of Botany and Plant Sciences, University of California, 900 University Avenue, Riverside, CA 92521, USA., Boukar O; International Institute of Tropical Agriculture (IITA), Kano, Nigeria.
Jazyk: angličtina
Zdroj: International journal of genomics [Int J Genomics] 2024 Jan 09; Vol. 2024, pp. 9912987. Date of Electronic Publication: 2024 Jan 09 (Print Publication: 2024).
DOI: 10.1155/2024/9912987
Abstrakt: Molecular markers are increasingly being deployed to accelerate genetic gain in crop plants. The objective of this study was to assess the potential of a mid-density genotyping panel for molecular applications in cowpea breeding. A core set of 2,602 targeted diversity array technology (DArTag) single-nucleotide polymorphisms (SNPs) was designed from an existing 51,128 Cowpea iSelect Consortium Array. The panel's usefulness was assessed using 376 genotypes from different populations of known genetic backgrounds. The panel was informative, with over 78% of SNPs exceeding a minor allele frequency of 0.20. The panel decoded three stratifications in the constituted population, as was expected. Linkage disequilibrium (LD) decay was correctly depicted as slower in a biparental subset than in other populations. A known flower and seed coat color gene region was located on chromosome Vu07, suggesting that the mid-density panel may be used to hypothesize genomic regions underlying target traits in cowpea. Unexpected heterozygosity was detected in some lines and highly among F 1 progenies, divulging the panel's potential application in germplasm purity and hybridity verification. The study unveils the potential of an excellent genomic resource that can be tapped to enhance the development of improved cowpea cultivars.
Competing Interests: The authors declare that they have no conflict of interest.
(Copyright © 2024 Patrick Obia Ongom et al.)
Databáze: MEDLINE
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