Protosequences in human cortical organoids model intrinsic states in the developing cortex.

Autor:	van der Molen T; Neuroscience Research Institute, University of California Santa Barbara, Santa Barbara, CA 93106, USA.; Department of Molecular, Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, CA 93106, USA., Spaeth A; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA 95060, USA.; Department of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, CA 95064, USA., Chini M; Institute of Developmental Neurophysiology, Center for Molecular Neurobiology, Hamburg Center of Neuroscience, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany., Bartram J; Department of Biosystems Science and Engineering, ETH Zürich, Klingelbergstrasse 48, 4056 Basel, Switzerland., Dendukuri A; Department of Computer Science, University of California Santa Barbara, Santa Barbara, CA 93106, USA., Zhang Z; Department of Physics, University of California Santa Barbara, Santa Barbara, CA 93106., Bhaskaran-Nair K; Department of Biology, Washington University in St. Louis, St. Louis, MO 63130, USA., Blauvelt LJ; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA 95060, USA., Petzold LR; Department of Computer Science, University of California Santa Barbara, Santa Barbara, CA 93106, USA., Hansma PK; Neuroscience Research Institute, University of California Santa Barbara, Santa Barbara, CA 93106, USA.; Department of Physics, University of California Santa Barbara, Santa Barbara, CA 93106., Teodorescu M; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA 95060, USA.; Department of Electrical and Computer Engineering, University of California Santa Cruz, Santa Cruz, CA 95064, USA., Hierlemann A; Department of Biosystems Science and Engineering, ETH Zürich, Klingelbergstrasse 48, 4056 Basel, Switzerland., Hengen KB; Department of Biology, Washington University in St. Louis, St. Louis, MO 63130, USA., Hanganu-Opatz IL; Institute of Developmental Neurophysiology, Center for Molecular Neurobiology, Hamburg Center of Neuroscience, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany., Kosik KS; Neuroscience Research Institute, University of California Santa Barbara, Santa Barbara, CA 93106, USA.; Department of Molecular, Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, CA 93106, USA., Sharf T; UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA 95060, USA.; Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA 95064, USA.; Institute for the Biology of Stem Cells, University of California Santa Cruz, Santa Cruz, CA 95064, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Dec 30. Date of Electronic Publication: 2023 Dec 30.
DOI:	10.1101/2023.12.29.573646
Abstrakt:	Neuronal firing sequences are thought to be the basic building blocks of neural coding and information broadcasting within the brain. However, when sequences emerge during neurodevelopment remains unknown. We demonstrate that structured firing sequences are present in spontaneous activity of human brain organoids and ex vivo neonatal brain slices from the murine somatosensory cortex. We observed a balance between temporally rigid and flexible firing patterns that are emergent phenomena in human brain organoids and early postnatal murine somatosensory cortex, but not in primary dissociated cortical cultures. Our findings suggest that temporal sequences do not arise in an experience-dependent manner, but are rather constrained by an innate preconfigured architecture established during neurogenesis. These findings highlight the potential for brain organoids to further explore how exogenous inputs can be used to refine neuronal circuits and enable new studies into the genetic mechanisms that govern assembly of functional circuitry during early human brain development. Competing Interests: Competing interests: All authors declare no competing interests.
Databáze:	MEDLINE
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