Single-cell transcriptomics of the immune system in ME/CFS at baseline and following symptom provocation.

Autor:	Vu LT; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA., Ahmed F; Genomics Innovation Hub and TREx Facility, Institute of Biotechnology, Cornell University, Ithaca, NY 14853, USA., Zhu H; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA., Iu DSH; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA., Fogarty EA; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA., Kwak Y; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA., Chen W; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA., Franconi CJ; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA., Munn PR; Genomics Innovation Hub and TREx Facility, Institute of Biotechnology, Cornell University, Ithaca, NY 14853, USA., Tate AE; Genomics Innovation Hub and TREx Facility, Institute of Biotechnology, Cornell University, Ithaca, NY 14853, USA., Levine SM; Levine Clinic, New York, NY, USA., Stevens J; Workwell Foundation, Ripon, CA, USA., Mao X; Department of Radiology, Weill Cornell Medicine, New York, NY, USA., Shungu DC; Department of Radiology, Weill Cornell Medicine, New York, NY, USA., Moore GE; Department of Exercise Science and Athletic Training, Ithaca College, Ithaca, NY, USA., Keller BA; Department of Exercise Science and Athletic Training, Ithaca College, Ithaca, NY, USA., Hanson MR; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA., Grenier JK; Genomics Innovation Hub and TREx Facility, Institute of Biotechnology, Cornell University, Ithaca, NY 14853, USA. Electronic address: jgrenier@cornell.edu., Grimson A; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA. Electronic address: agrimson@cornell.edu.
Jazyk:	angličtina
Zdroj:	Cell reports. Medicine [Cell Rep Med] 2024 Jan 16; Vol. 5 (1), pp. 101373.
DOI:	10.1016/j.xcrm.2023.101373
Abstrakt:	Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a serious and poorly understood disease. To understand immune dysregulation in ME/CFS, we use single-cell RNA sequencing (scRNA-seq) to examine immune cells in patient and control cohorts. Postexertional malaise (PEM), an exacerbation of symptoms following strenuous exercise, is a characteristic symptom of ME/CFS. To detect changes coincident with PEM, we applied scRNA-seq on the same cohorts following exercise. At baseline, ME/CFS patients display classical monocyte dysregulation suggestive of inappropriate differentiation and migration to tissue. We identify both diseased and more normal monocytes within patients, and the fraction of diseased cells correlates with disease severity. Comparing the transcriptome at baseline and postexercise challenge, we discover patterns indicative of improper platelet activation in patients, with minimal changes elsewhere in the immune system. Taken together, these data identify immunological defects present at baseline in patients and an additional layer of dysregulation in platelets. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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