| Autor: |
Montoya AM; Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Ave. Francisco I. Madero s/n, Monterrey, 64460, México., Rodríguez-Grimaldo JE; Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Ave. Francisco I. Madero s/n, Monterrey, 64460, México., López-Jácome LE; Laboratorio de Infectología, Instituto Nacional de Rehabilitación 'Luis Guillermo Ibarra Ibarra', Mexico City, 14389, Mexico., Bonifaz A; Servicio de Dermatología y Departamento de Microbiología, Hospital General de México 'Dr. Eduardo Liceaga', Mexico City, 06720, Mexico., Enríquez-Domínguez E; Laboratorio de Micología Experimental, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, San Luis Potosi, 78210, Mexico., Castañón-Olivares LR; Departamento de Microbiología y Parasitología, Universidad Nacional Autónoma de México, Mexico City, 04510, Mexico., Charles-Niño CL; Departamento de Microbiología y Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, 44340, Mexico., Rodríguez-Rodríguez A; Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, San Luis Potosi, 78210, Mexico., de J Treviño-Rangel R; Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Ave. Francisco I. Madero s/n, Monterrey, 64460, México., Rojas OC; Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Ave. Francisco I. Madero s/n, Monterrey, 64460, México., González GM; Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Ave. Francisco I. Madero s/n, Monterrey, 64460, México. |
| Abstrakt: |
Fusarium spp. has emerged as an opportunistic etiological agent with clinical manifestations varying from localized infections to deep-seated systemic disease. It is also a phytopathogen of economic impact. There are few reports on the species diversity of this genus, and no comprehensive studies on the epidemiology nor the antifungal susceptibility of Fusarium in Mexico. The present multicentric study aims to shed light on the species distribution and antifungal susceptibility patterns of 116 strains of Fusarium isolated from clinical and environmental samples. Isolates were identified by standard phenotypic characteristics and by sequencing of the ITS (internal transcribed spacer), TEF1 (translation elongation factor 1-α), RPB2 (RNA polymerase II core subunit), and/or CAM1 (calmodulin) regions. Susceptibility tests were carried out against 15 antifungals of clinical and agricultural use. Regarding Fusarium distribution, we identified 27 species belonging to eight different species complexes. The most frequently isolated species for both clinical and environmental samples were F. falciforme (34%), F. oxysporum sensu stricto (12%), F. keratoplasticum (8%), and F. solani sensu stricto (8%). All Fusarium isolates showed minimum inhibitory concentrations (MICs) equal to or above the maximum concentration evaluated for fluconazole, 5-fluocytosine, caspofungin, micafungin, and anidulafungin. All isolates had a MIC of ≤16 µg/mL for voriconazole, with a mode of 4 µg/mL. F. verticillioides appeared to be the most susceptible to all antifungals tested. |
