| Autor: |
Lisowski MD; M.V. Lomonosov Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 119571 Moscow, Russia., Korobova EV; M.V. Lomonosov Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 119571 Moscow, Russia., Naumova AO; M.V. Lomonosov Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 119571 Moscow, Russia., Sedishev IP; M.V. Lomonosov Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 119571 Moscow, Russia., Markova AA; Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygin Street, 119334 Moscow, Russia.; Institute of Cyber Intelligence Systems, National Research Nuclear University MEPhI, 115409 Moscow, Russia., Nguyen MT; Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygin Street, 119334 Moscow, Russia., Kuzmin VA; Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygin Street, 119334 Moscow, Russia., Nichugovskiy AI; M.V. Lomonosov Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 119571 Moscow, Russia., Arlyapov VA; Research Center 'BioChemTech', Tula State University, 92 Lenin Avenue, 300012 Tula, Russia., Yashtulov NA; M.V. Lomonosov Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 119571 Moscow, Russia., Melnikov PV; M.V. Lomonosov Institute of Fine Chemical Technologies, MIREA-Russian Technological University, 119571 Moscow, Russia. |
| Abstrakt: |
The use of biocidal agents is a common practice for protection against biofouling in biomass-rich environments. In this paper, oligohexamethyleneguanidine (OHMG) polymer, known for its biocidal properties, was further modified with para-aminosalicylic acid (PAS) to enhance its properties against microorganisms coated with a lipid membrane. The structure of the product was confirmed by 1 H NMR, 13 C NMR, and FTIR spectroscopy. The values of the minimum inhibitory concentration (MIC) against Mycobacterium smegmatis ATCC 607 and Pseudomonas chlororaphis 449 were found to be 1.40 and 1.05 μg/mL, respectively. The synthesized substance was used as an additive to the polymer matrix of the composite optical oxygen sensor material. A series of samples with different contents of OHMG-PAS was prepared using a co-dissolution method implying the fabrication of a coating from a solution containing both polymers. It turned out that the mutual influence of the components significantly affects the distribution of the indicator in the matrix, surface morphology, and contact angle. The optimal polymer content turned out to be wt.3%, at which point the water contact angle reaches almost 122°, and the fouling rate decreases by almost five times, which is confirmed by both the respiratory MTT assay and confocal microscopy with staining. This opens up prospects for creating stable and biofouling-resistant sensor elements for use in air tanks or seawater. |
