Metabolic and transcriptomic characterization of summer and winter dormancy in the solitary bee, Osmia lignaria.

Autor: Melicher D; Edward T. Schafer Agricultural Research Center, U.S. Department of Agriculture/Agricultural Research Service, 1616 Albrecht Boulevard North, Fargo, ND, 58102, USA., Torson AS; Edward T. Schafer Agricultural Research Center, U.S. Department of Agriculture/Agricultural Research Service, 1616 Albrecht Boulevard North, Fargo, ND, 58102, USA. Electronic address: Alex.Torson@usda.gov., Yocum GD; Edward T. Schafer Agricultural Research Center, U.S. Department of Agriculture/Agricultural Research Service, 1616 Albrecht Boulevard North, Fargo, ND, 58102, USA., Bosch J; CREAF, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain., Kemp WP; Edward T. Schafer Agricultural Research Center, U.S. Department of Agriculture/Agricultural Research Service, 1616 Albrecht Boulevard North, Fargo, ND, 58102, USA., Bowsher JH; Department of Biological Sciences, North Dakota State University, 1340 Bolley Drive, 218 Stevens Hall, Fargo, ND, 58102, USA., Rinehart JP; Edward T. Schafer Agricultural Research Center, U.S. Department of Agriculture/Agricultural Research Service, 1616 Albrecht Boulevard North, Fargo, ND, 58102, USA.
Jazyk: angličtina
Zdroj: Insect biochemistry and molecular biology [Insect Biochem Mol Biol] 2024 Mar; Vol. 166, pp. 104074. Date of Electronic Publication: 2024 Jan 14.
DOI: 10.1016/j.ibmb.2024.104074
Abstrakt: The solitary bee Osmia lignaria is a native pollinator in North America with growing economic importance. The life cycle of O. lignaria provides a unique opportunity to compare the physiological and molecular mechanisms underlying two ecologically contrasting dormancies within the same species. O. lignaria prepupae become dormant during the summer to avoid high temperatures. Shortly after adult eclosion, they enter a second dormancy and overwinter as diapausing adults. To compare these two dormancies, we measured metabolic rates and gene expression across development as bees initiate, maintain, and terminate both prepupal (summer) and adult (overwintering) dormancies. We observed a moderate temperature-independent decrease in gas exchange during both the prepupal dormancy after cocoon spinning (45 %) and during adult diapause after eclosion (60 %). We sequenced and assembled a high-quality reference genome from a single haploid male bee with a contiguous n50 of 5.5 Mbp to facilitate our transcriptomic analysis. The transcriptomes of dormant prepupae and diapausing adults clustered into distinct groups more closely associated with life stage than dormancy status. Membrane transport, membrane-bound cellular components, oxidoreductase activity, glutathione metabolism, and transcription factor activity increased during adult diapause, relative to prepupal dormancy. Further, the transcriptomes of adults in diapause clustered into two groups, supporting multiple phases of diapause during winter. Late adult diapause was associated with gene expression profiles supporting increased insulin/IGF, juvenile hormone, and ecdysone signaling.
Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.
(Published by Elsevier Ltd.)
Databáze: MEDLINE
Externí odkaz: