Biomedical and ecosafety assessment of marine fish collagen capped silver nanoparticles.
| Autor: | Vijayakumar S; Marine College, Shandong University, Weihai 264209, PR China. Electronic address: sekarvijayakumar@sdu.edu.cn., Chen J; Marine College, Shandong University, Weihai 264209, PR China. Electronic address: jdchen@sdu.edu.cn., González-Sánchez ZI; Nanobiology Laboratory, Department of Natural and Exact Sciences, Pontificia Universidad Católica Madre y Maestra, PUCMM, Autopista Duarte Km 1 ½, Santiago de los Caballeros, Dominican Republic; Department of Medical Biochemistry, Molecular Biology and Immunology, School of Medicine, University of Seville, Seville, Spain., Tungare K; School of Biotechnology and Bioinformatics, D. Y. Patil Deemed to be University, CBD Belapur, Plot No-50, Sector-15, Navi Mumbai 400614, India; Anatek Services PVT Ltd, 10, Sai Chamber, Near Santacruz Railway Bridge, Sen Nagar, Santacruz East, Mumbai, Maharashtra 400055, India. Electronic address: dr.kanchanlata19@gmail.com., Bhori M; School of Biotechnology and Bioinformatics, D. Y. Patil Deemed to be University, CBD Belapur, Plot No-50, Sector-15, Navi Mumbai 400614, India; Invenio life Technology PVT Ltd, Office No.118, Grow More Tower, Plot No.5, Sector 2, Kharghar, Navi Mumbai, Maharashtra 410210, India., Shakila H; Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India., Sruthi KS; Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India., Divya M; BioMe-Live Analytical Centre, Karaikudi, Tamil Nadu, India., Durán-Lara EF; Bio&NanoMaterialsLab, Drug Delivery and Controlled Release, Universidad de Talca, Talca 3460000, Maule, Chile; Departamento de Microbiología, Facultad de Ciencias de la Salud, Universidad de Talca, Talca 3460000, Maule, Chile., Thandapani G; PG and Research Department of Chemistry, D.K.M. College for Women (Autonomous), Affiliated to Thiruvalluvar University, Vellore, Tamil Nadu, India., Anbu P; Department of Biological Engineering, Inha University, Incheon 402-751, Republic of Korea. Electronic address: anbu25@yahoo.com. |
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| Jazyk: | angličtina |
| Zdroj: | International journal of biological macromolecules [Int J Biol Macromol] 2024 Mar; Vol. 260 (Pt 1), pp. 129324. Date of Electronic Publication: 2024 Jan 14. |
| DOI: | 10.1016/j.ijbiomac.2024.129324 |
| Abstrakt: | In the rapidly evolving landscape of silver nanoparticles (Ag NPs) synthesis, the focus has predominantly been on plant-derived sources, leaving the realm of biological or animal origins relatively uncharted. Breaking new ground, our study introduces a pioneering approach: the creation of Ag NPs using marine fish collagen, termed ClAg NPs, and offers a comprehensive exploration of their diverse attributes. To begin, we meticulously characterized ClAg NPs, revealing their spherical morphology, strong crystalline structure, and average diameter of 5 to 100 nm. These NPs showed potent antibacterial activity, notably against S. aureus (gram-positive), surpassing their efficacy against S. typhi (gram-negative). Additionally, ClAg NPs effectively hindered the growth of MRSA biofilms at 500 μg/mL. Impressively, they demonstrated substantial antioxidant capabilities, out performing standard gallic acid. Although higher concentrations of ClAg NPs induced hemolysis (41.804 %), lower concentrations remained non hemolytic. Further evaluations delved into the safety and potential applications of ClAg NPs. In vitro cytotoxicity studies on HEK 293 and HeLa cells revealed dose-dependent toxicity, with IC Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| Databáze: | MEDLINE |
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