| Autor: |
Zhao S; College of Plant Science, Jilin University, Changchun, China., Yan F; College of Plant Science, Jilin University, Changchun, China., Liu Y; College of Plant Science, Jilin University, Changchun, China., Sun M; College of Plant Science, Jilin University, Changchun, China., Wang Y; College of Plant Science, Jilin University, Changchun, China., Li J; College of Plant Science, Jilin University, Changchun, China., Zhang X; College of Plant Science, Jilin University, Changchun, China., Yang X; College of Plant Science, Jilin University, Changchun, China., Wang Q; College of Plant Science, Jilin University, Changchun, China. |
| Abstrakt: |
The fatty acyl-acyl carrier protein thioesterase B (FATB ) gene, involved in the synthesis of saturated fatty acids, plays an important role in the content of fatty acid and composition of seed storage lipids. However, the role of FATB in soybeans (Glycine max ) has been poorly characterised. This paper presents a preliminary bioinformatics and molecular biological investigation of 10 hypothetical FATB members. The results revealed that GmFATB1B , GmFATB2A and GmFATB2B contain many response elements involved in defense and stress responses and meristem tissue expression. Moreover, the coding sequences of GmFATB1A and GmFATB1B were significantly longer than those of the other genes. Their expression varied in different organs of soybean plants during growth, with GmFATB2A and GmFATB2B showing higher relative expression. In addition, subcellular localisation analysis revealed that they were mainly present in chloroplasts. Overexpression of GmFATB1A , GmFATB1B , GmFATB2A and GmFATB2B in transgenic Arabidopsis thaliana plants increased the seed oil content by 10.3%, 12.5%, 7.5% and 8.4%, respectively, compared to that in the wild-type and led to significant increases in palmitic and stearic acid content. Thus, this research has increased our understanding of the FATB family in soybeans and provides a theoretical basis for subsequent improvements in soybean quality. |
