Isothermal amplification methods in cancer-related miRNA detection; a new paradigm in study of cancer pathology.

Autor: Al-Hawary SIS; Department of Business Administration, Business School, Al al-Bayt University, P.O. BOX 130040, Mafraq 25113, Jordan., Saleh RO; Department of Medical Laboratory Techniques, Al-Maarif University College, Al-Anbar, Iraq., Mansouri S; Department of Biomedical Technology, College of Applied Medical Sciences in Al-Kharj, Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia; University of Tunis El Manar, Higher Institute of Medical Technologies of Tunis, Laboratory of Biophysics and Medical Technologies, Tunis, Tunisia. Electronic address: s.mansouri@psau.edu.sa., Noraldeen SAM; Medical Laboratory Technology, University of Tabuk, Tabuk, Saudi Arabia. Electronic address: snoraldeen@ut.edu.sa., Alawadi AH; College of Technical Engineering, University of Najaf, Najaf, Iraq; College of Technical Engineering, the Islamic University of Al Diwaniyah, Iraq; College of Technical Engineering, the Islamic University of Babylon, Iraq., Kareem AH; College of Health and Medical Technology, Al-Ayen University, Thi-Qar 64001, Iraq., Alsalamy A; College of Technical Engineering, Imam Ja'afar Al-Sadiq University, Al-Muthanna 66002, Iraq., Shakir MN; Department of Medical Laboratories Technology, AL-Nisour University College, Baghdad, Iraq., Alasheqi MQ; College of Pharmacy, National University of Science and Technology, Dhi Qar, Iraq., Mustafa YF; Department of Pharmaceutical Chemistry, College of Pharmacy, University of Mosul, Mosul 41001, Iraq.
Jazyk: angličtina
Zdroj: Pathology, research and practice [Pathol Res Pract] 2024 Feb; Vol. 254, pp. 155072. Date of Electronic Publication: 2023 Dec 30.
DOI: 10.1016/j.prp.2023.155072
Abstrakt: MicroRNAs (miRNAs) are short, non-coding RNA molecules that regulate gene expression. They are involved in a wide range of biological processes, including development, differentiation, cell cycle regulation, and response to stress. Numerous studies have demonstrated that miRNAs are present in different bodily fluids, which could serve as an important biomarker. The advancement of techniques and strategies for the identification of cancer-associated miRNAs in human specimens offers a novel opportunity to diagnose cancer in early stages, predict patient prognosis and evaluate response to treatment. Isothermal techniques including loop-mediated isothermal amplification (LAMP), rolling circle amplification (RCA), or recombinase polymerase amplification (RPA) offer simplicity, efficiency, and rapidity in miRNA detection processes. In contrast to traditional PCR (polymerase chain reaction), these techniques analysis and quantify miRNA molecules in specimens using a single constant temperature. In this comprehensive review, we summarized the recent advances in cancer-related miRNA detection via highly sensitive isothermal amplification methods by more focusing on the involved mechanism.
Competing Interests: Declaration of Competing Interest I declare that there is no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: