A conserved transcription factor controls gluconeogenesis via distinct targets in hypersaline-adapted archaea with diverse metabolic capabilities.

Autor: Hackley RK; Department of Biology, Duke University, Durham, North Carolina, United States of America.; University Program in Genetics and Genomics, Duke University, Durham, North Carolina, United States of America., Vreugdenhil-Hayslette A; Department of Biology, Duke University, Durham, North Carolina, United States of America., Darnell CL; Department of Biology, Duke University, Durham, North Carolina, United States of America., Schmid AK; Department of Biology, Duke University, Durham, North Carolina, United States of America.; University Program in Genetics and Genomics, Duke University, Durham, North Carolina, United States of America.; Center for Genomics and Computational Biology, Duke University, Durham, North Carolina, United States of America.
Jazyk: angličtina
Zdroj: PLoS genetics [PLoS Genet] 2024 Jan 16; Vol. 20 (1), pp. e1011115. Date of Electronic Publication: 2024 Jan 16 (Print Publication: 2024).
DOI: 10.1371/journal.pgen.1011115
Abstrakt: Timely regulation of carbon metabolic pathways is essential for cellular processes and to prevent futile cycling of intracellular metabolites. In Halobacterium salinarum, a hypersaline adapted archaeon, a sugar-sensing TrmB family protein controls gluconeogenesis and other biosynthetic pathways. Notably, Hbt. salinarum does not utilize carbohydrates for energy, uncommon among Haloarchaea. We characterized a TrmB-family transcriptional regulator in a saccharolytic generalist, Haloarcula hispanica, to investigate whether the targets and function of TrmB, or its regulon, is conserved in related species with distinct metabolic capabilities. In Har. hispanica, TrmB binds to 15 sites in the genome and induces the expression of genes primarily involved in gluconeogenesis and tryptophan biosynthesis. An important regulatory control point in Hbt. salinarum, activation of ppsA and repression of pykA, is absent in Har. hispanica. Contrary to its role in Hbt. salinarum and saccharolytic hyperthermophiles, TrmB does not act as a global regulator: it does not directly repress the expression of glycolytic enzymes, peripheral pathways such as cofactor biosynthesis, or catabolism of other carbon sources in Har. hispanica. Cumulatively, these findings suggest rewiring of the TrmB regulon alongside metabolic network evolution in Haloarchaea.
Competing Interests: The authors have no competing interests.
(Copyright: © 2024 Hackley et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje