MicroRNAs correlate with bacillary index and genes associated to cell death processes in leprosy.

Autor:	de Lima Santana N; Programa de Pós-graduação em Ciências da Saúde da Universidade Federal da Bahia, Salvador, Brazil; Serviço de Imunologia da Universidade Federal da Bahia, Salvador, Brazil. Electronic address: nlimas@hotmail.com., Neves de Farias L; Programa de Pós-graduação em Ciências da Saúde da Universidade Federal da Bahia, Salvador, Brazil; Serviço de Imunologia da Universidade Federal da Bahia, Salvador, Brazil. Electronic address: nfarias.lucas@hotmail.com., Lago T; Programa de Pós-graduação em Ciências da Saúde da Universidade Federal da Bahia, Salvador, Brazil; Serviço de Imunologia da Universidade Federal da Bahia, Salvador, Brazil. Electronic address: tataylago@gmail.com., Leal-Calvo T; Laboratório de Hanseníase, Fundação Oswaldo Cruz-FIOCRUZ, Rio de Janeiro, Brazil. Electronic address: thyagoleal@yahoo.com., Lima Machado PR; Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais, Brazil; Programa de Pós-graduação em Ciências da Saúde da Universidade Federal da Bahia, Salvador, Brazil; Serviço de Imunologia da Universidade Federal da Bahia, Salvador, Brazil. Electronic address: prlmachado@uol.com.br., Castellucci LC; Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais, Brazil; Programa de Pós-graduação em Ciências da Saúde da Universidade Federal da Bahia, Salvador, Brazil; Serviço de Imunologia da Universidade Federal da Bahia, Salvador, Brazil. Electronic address: leacastel@hotmail.com.
Jazyk:	angličtina
Zdroj:	Microbes and infection [Microbes Infect] 2024 May-Jun; Vol. 26 (4), pp. 105300. Date of Electronic Publication: 2024 Jan 14.
DOI:	10.1016/j.micinf.2024.105300
Abstrakt:	Mycobacterium leprae infects skin and peripheral nerves causing a broad of clinical forms. MicroRNAs (miRNAs) control immune mechanisms such as apoptosis, autophagy as well as to target genes leading to abnormal proliferation, metastasis, and invasion of cells. Herein we evaluated miRNAs expression for leprosy phenotypes in biopsies obtained from patients with and without reactions. We also correlated those miRNAs with both, bacillary index (BI) and genes involved in the micobacteria elimination process. Our results show a significant increase in the miR-125a-3p expression in paucibacillary (PB) patients vs multibacillary (MB) subjects (p = 0.007) and vs reversal reactions (RR) (p = 0.005), respectively. Likewise, there was a higher expression of miR-125a-3p in patients with erythema nodosum leprosum (ENL) vs MB without reactions (p = 0.002). Furthermore, there was a positive correlation between miR-125a-3p, miR-146b-5p and miR-132-5p expression and BI in patients with RR and ENL. These miRNAS were also correlated with genes such as ATG12 (miR-125a-3p), TNFRSF10A (miR-146b-5p), PARK2, CFLAR and STX7 (miR-132-5p). All together we underpin a role for these miRNAs in leprosy pathogenesis, implicating mechanisms such as apoptosis and autophagy in skin. The miR-125a-3p might have a distinct role associated with PB phenotype and ENL in MB patients. Competing Interests: Declaration of competing interest The authors declare that they have no competing interests. (Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Full Text from ScienceDirect