Molecular monitoring of treatment efficacy in human visceral leishmaniasis.

Autor: Roy S; Department of Pharmacology, Institute of Postgraduate Medical Education and Research, Kolkata 700020, India., Moulik S; Department of Pharmacology, Institute of Postgraduate Medical Education and Research, Kolkata 700020, India., Chaudhuri SJ; Department of Microbiology, Sarat Chandra Chattopadhyay Government Medical College and Hospital, Uluberia, Howrah 711316, India., Ghosh MK; Department of Tropical Medicine, School of Tropical Medicine, Kolkata700073, India., Goswami RP; Department of Tropical Medicine, School of Tropical Medicine, Kolkata700073, India., Saha B; Department of Tropical Medicine, School of Tropical Medicine, Kolkata700073, India., Chatterjee M; Department of Pharmacology, Institute of Postgraduate Medical Education and Research, Kolkata 700020, India.
Jazyk: angličtina
Zdroj: Transactions of the Royal Society of Tropical Medicine and Hygiene [Trans R Soc Trop Med Hyg] 2024 May 01; Vol. 118 (5), pp. 343-345.
DOI: 10.1093/trstmh/trad103
Abstrakt: Background: Focused efforts of the visceral leishmaniasis elimination program have led to a drastic decline in cases, and the present challenge is disease monitoring, which this study aimed to assess.
Methods: A Leishmania kinetoplastid-targeted qPCR quantified parasite load at disease presentation, and following treatment completion (n=49); an additional 80 cases were monitored after completion of treatment.
Results: The parasite load at disease presentation was 13 461.00 (2560.00-37764.00)/µg gDNA, which upon completion of treatment reduced in 47 of 49 cases to 1(1-1)/µg gDNA, p<0.0001. In 80 cases that presented >2 months post-treatment, their parasite burden similarly decreased to 1(1-1)/µg gDNA except in 6 of 80 cases, which were qPCR positive.
Conclusion: In 129 cases of visceral leishmaniasis, qPCR by quantification of parasite burden proved effective for monitoring treatment.
(© The Author(s) 2024. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene.)
Databáze: MEDLINE
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