A fluorescence-based immunochromatographic assay using quantum dot-encapsulated nanoparticles for the rapid and sensitive detection of fetuin-B.

Autor: Hou J; Center for Cardiovascular Diseases, Meizhou Clinical Institute of Shantou University Medical College, Meizhou, Guangdong, 514031, China; GuangDong Engineering Technology Research Center for Molecular Diagnostics of Cardiovascular Diseases, Meizhou, Guangdong, 514031, China., Cao Y; Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, 510180, China., Deng Q; Center for Cardiovascular Diseases, Meizhou Clinical Institute of Shantou University Medical College, Meizhou, Guangdong, 514031, China., Zhang Q; Center for Cardiovascular Diseases, Meizhou Clinical Institute of Shantou University Medical College, Meizhou, Guangdong, 514031, China., Deng X; Center for Cardiovascular Diseases, Meizhou Clinical Institute of Shantou University Medical College, Meizhou, Guangdong, 514031, China., Chen Z; Department of Laboratory Medicine, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, 510180, China. Electronic address: zhongzhixiong@mzrmyy.com., Zhong Z; Center for Cardiovascular Diseases, Meizhou Clinical Institute of Shantou University Medical College, Meizhou, Guangdong, 514031, China; GuangDong Engineering Technology Research Center for Molecular Diagnostics of Cardiovascular Diseases, Meizhou, Guangdong, 514031, China. Electronic address: czh56248@126.com.
Jazyk: angličtina
Zdroj: Analytica chimica acta [Anal Chim Acta] 2024 Feb 01; Vol. 1288, pp. 342143. Date of Electronic Publication: 2023 Dec 15.
DOI: 10.1016/j.aca.2023.342143
Abstrakt: Coronary artery disease (CAD) is the leading cause of death worldwide. Earlier detection of CAD improves treatment outcomes and secondary prevention. The circulating fetuin-B protein is considered to be a promising biomarker for the early detection of CAD. However, a facile and reliable clinical test for fetuin-B is still lacking. Herein, we describe a reliable fluorescent biosensor for detecting fetuin-B in plasma that combines quantum dots-doped polystyrene nanoparticles with an immunochromatographic assay strip (QNPs-ICAS). The QNPs served as detection signals in the QNPs-ICAS sensor system, which was based on a double-antibody sandwich structure. Under optimum experimental conditions, the biosensor exhibited a broad linear range of 1-200 ng mL -1 and a low detection limit of 0.299 ng mL -1 . Furthermore, the proposed immunosensor demonstrated high sensitivity, satisfactory selectivity, good reproducibility, and excellent recovery. Finally, the performance and applicability of our QNPs-based ICAS system were validated in clinical samples using a commercial ELISA kit with excellent correlations (r = 0.98451, n = 116). To conclude, the proposed sensor served as a rapid, sensitive, and accurate method for detecting fetuin-B in actual clinical samples, thereby demonstrating its potential for preliminary CAD screening and diagnosis.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: