Protein-polysaccharide based double network microbeads improves stability of Bifidobacterium infantis ATCC 15697 in a gastro-Intestinal tract model (TIM-1).

Autor: Khan WA; Department of Food Science and Technology, University of Home Economics Lahore, 54660 Pakistan. Electronic address: w.a.khan31@hotmail.com., Butt MS; National Institute of Food Science & Technology, Faculty of Food, Nutrition & Home Sciences, University of Agriculture Faisalabad, 38040 Pakistan. Electronic address: drmsbutt@uaf.edu.pk., Yasmin I; Department of Human Nutrition and Dietetics, University of Chakwal, Chakwal, 48800 Pakistan. Electronic address: iqra.yasmin@uoc.edu.pk., Wadood SA; Department of Food Science and Technology, University of Home Economics Lahore, 54660 Pakistan; Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture and Rural Affairs of China, Hangzhou 310021, China. Electronic address: abwadood.fn@uhe.edu.pk., Mahmood A; Department of Pharmacy, University of Chakwal, Chakwal 48800, Pakistan. Electronic address: asif.mahmood@uoc.edu.pk., Gad HA; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo 11566, Egypt; Department of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, Jeddah 21442, Saudi Arabia. Electronic address: h.gad@pharma.asu.edu.eg.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2024 Mar 05; Vol. 652, pp. 123804. Date of Electronic Publication: 2024 Jan 12.
DOI: 10.1016/j.ijpharm.2024.123804
Abstrakt: Microencapsulation of probiotics is a main technique employed to improve cell survival in gastrointestinal tract (GIT). The present study investigated the impact of utilizing proteins i.e. Whey Protein Isolates (WPI), Pea Protein Isolates (PPI) or (WPI + PPI) complex based microbeads as encapsulating agents on the encapsulation efficiency (EE), diameter, morphology along with the survival and viability of Bifidobacterium infantis ATCC 15697. Results revealed that WPI + PPI combination had the highest EE% of the probiotics up to 94.09 % and the smoothest surface with less visible holes. WPI based beads revealed lower EE% and smaller size than PPI based ones. In addition, WPI based beads showed rough surface with visible signs of cracks, while PPI beads showed dense surfaces with pores and depressions. In contrast, the combination of the two proteins resulted in compact and smooth beads with less visible pores/wrinkles. The survival in gastrointestinal tract (GIT) was observed through TNO in-vitro gastrointestinal model (TIM-1) and results illustrated that all microbeads shrank in gastric phase while swelled in intestinal phase. In addition, in-vitro survival rate of free cells was very low in gastric phase (18.2 %) and intestinal phase (27.5 %). The free cells lost their viability after 28 days of storage (2.66 CFU/mL) with a maximum log reduction of 6.76, while all the encapsulated probiotic showed more than 10 6-7 log CFU/g viable cell. It was concluded that encapsulation improved the viability of probiotics in GIT and utilization of WPI + PPI in combination provided better protection to probiotics.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: