The effects of induced type I diabetes on developmental regulation of GDNF, NRTN, and NCAM proteins in the dentate gyrus of male rat offspring.

Autor: Mostafaee H; Department of Anatomical Sciences, Birjand University of Medical Sciences, Iran., Idoon F; Department of Anatomical Sciences, Birjand University of Medical Sciences, Iran., Mohasel-Roodi M; Department of Anatomical Sciences, Birjand University of Medical Sciences, Iran., Alipour F; Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Lotfi N; Department of Anatomical Sciences, Birjand University of Medical Sciences, Iran., Sadeghi A; Department of Anatomical Sciences, Birjand University of Medical Sciences, Iran; Institute of Anatomy and Molecular Neurobiology, Westfälische Wilhelms-University, Münster, Germany. Electronic address: akram.sadeghi@uni-muenster.de.
Jazyk: angličtina
Zdroj: Journal of chemical neuroanatomy [J Chem Neuroanat] 2024 Mar; Vol. 136, pp. 102391. Date of Electronic Publication: 2024 Jan 14.
DOI: 10.1016/j.jchemneu.2024.102391
Abstrakt: Background: Maternal diabetes during pregnancy can affect the neurological development of offspring. Glial cell-derived neurotrophic factor (GDNF), neurturin (NRTN), and neural cell adhesion molecules (NCAM) are three important proteins for brain development. Therefore, this study aimed to investigate the impacts of the mentioned neurotrophic factors in the hippocampal dentate gyrus (DG) of rat offspring born to diabetic mothers.
Methods: Wistar female rats were randomly allocated into diabetic (STZ-D) [(45 mg/kg BW, STZ (Streptozotocin), i.p)], diabetic + NPH insulin (STZ-INS) [(4-6 unit/kg/day SC)], and control groups. The animals in all groups were mated by non-diabetic male rats. Two weeks after birth, male pups from each group were sacrificed and then protein contents of GDNF, NRTN, and NCAM were evaluated using immunohistochemistry.
Results: The study found that the expression of GDNF and NRTN in the hippocampus of diabetic rat offspring was significantly higher compared to the diabetic+ insulin and control groups, respectively (P < 0.01, P < 0.001). Additionally, the expression of NCAM was significantly higher in the diabetic group the diabetic+ insulin and control groups (P < 0.01, P < 0.001).
Conclusions: The results of the study revealed that diabetes during pregnancy significantly impacts the distribution pattern of GDNF, NRTN, and NCAM in the hippocampus of rat neonates.
Competing Interests: Declaration of Competing Interest The authors declare that there is no conflict of interest regarding the publication of this article.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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