A reliable and selective ratiometric sensing probe for fluorometric determination of P 2 O 7 4- based on AIE of GSH@CuNCs-assisted by Al-N@CQDs.

Autor: Mahmoud AM; Department of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran, Saudi Arabia., Abu-Alrub SS; Department of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran, Saudi Arabia., Al-Qarni AO; Department of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran, Saudi Arabia., El-Wekil MM; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt. Electronic address: mohamed.elwakeel@pharm.aun.edu.eg., Shahin RY; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sphinx University, New Assiut City, Assiut, Egypt.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2024 Apr 05; Vol. 310, pp. 123850. Date of Electronic Publication: 2024 Jan 09.
DOI: 10.1016/j.saa.2024.123850
Abstrakt: In this study, a novel composite material was developed for the ratiometric detection of pyrophosphate anion (P 2 O 7 4- ). This composite consisted of Al and nitrogen co-doped carbon dots (Al-N@CQDs) and glutathione-capped copper nanoclusters (GSH@CuNCs). The Al-N@CQDs component, with its high reserved coordination capacity of Al 3+ , induced the non-luminescent behavior of GSH@CuNCs, resulting in an aggregation-induced emission (AIE) effect. The hybrid material (Al-N@CQDs/GSH@CuNCs) exhibited dual-emission signals at 620 nm and 450 nm after integrating the two independent materials utilizing the AIE effect and the fluorescence resonance energy transfer (FRET) approach. This approach represents the first utilization of this composite for ratiometric detection. Nevertheless, upon the addition of P 2 O 7 4- , the AIE and FRET processes were hindered due to the higher coordination interaction of Al 3+ towards P 2 O 7 4- compared to the amino/carboxyl groups on Al-N@CQDs. This successful interference of the AIE and FRET processes allowed for the effective estimation of P 2 O 7 4- . The response ratio (F 450 /F 620 ) increased with increasing the concentration of P 2 O 7 4- in the range of 0.035-160 µM, with an impressive detection limit of 0.012 µM. This innovative approach of utilizing hybrid CQDs/thiolate-capped nanoclusters as a ratiometric fluorescent sensor for analytical applications introduces new possibilities in the field. The as-fabricated system was successfully applied to detect P 2 O 7 4- in different real samples such as water, serum, and urine samples with acceptable results.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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