Genomic diversity of β-lactamase producing Pseudomonas aeruginosa in Iran; the impact of global high-risk clones.

Autor: Ahmadi N; Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran., Salimizand H; Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran. hsalimizand@gmail.com., Zomorodi AR; Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran., Abbas JE; Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran., Ramazanzadeh R; Department of Microbiology, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran., Haghi F; Department of Microbiology, Zanjan University of Medical Sciences, Zanjan, Iran., Hassanzadeh S; Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Jahantigh M; Department of Microbiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran., Shahin M; Department of Medical Laboratory Sciences, Faculty of Medical Sciences, Arak branch, Islamic Azad University, Arak, Iran.
Jazyk: angličtina
Zdroj: Annals of clinical microbiology and antimicrobials [Ann Clin Microbiol Antimicrob] 2024 Jan 13; Vol. 23 (1), pp. 5. Date of Electronic Publication: 2024 Jan 13.
DOI: 10.1186/s12941-024-00668-5
Abstrakt: Background: Hospital-acquired infections caused by multidrug-resistant Pseudomonas aeruginosa incline hospital stay and costs of treatment that resulted in an increased mortality rate. The frequency of P. aeruginosa high-risk clones producing carbapenemases was investigated in our clinical samples.
Methods: In this cross-sectional study, 155 non-repetitive P. aeruginosa isolates were included from different medical centers of Iran. Antibiotic susceptibility testing was determined, and the presence of β-lactamases were sought by phenotypic and genotypic methods. The clonal relationship of all isolates was investigated, and multi-locus sequence typing (MLST) was used for finding the sequence types of carbapenemase-producers.
Results: The agent with highest percent susceptibility rate was recorded for colistin (94.9%). MOX and FOX were found both as low as 1.95% (3/155). The most frequent narrow spectrum β-lactamase was SHV with 7.7% (12/155) followed by PER, OXA-1, and TEM with the frequency of 7.1% (11/155), 3.2% (5/155), and 1.3% (2/155), respectively. Carbapenemases were detected in 28 isolates (18%). The most frequent carbapenemase was IMP with 9% (14/155) followed by NDM, 8.4% (13/155). OXA-48 and VIM were also detected both per one isolate (0.65%). MLST of carbapenem resistant P. aeruginosa isolates revealed that ST244, ST664, ST235, and ST357 were spread in subjected clinical settings. REP-PCR uncovered high genomic diversity in our clinical setting.
Conclusion: Clonal proliferation of ST235 strain plays a key role in the propagation of MDR pattern in P. aeruginosa. Our data showed that high-risk clones has distributed in Iran, and programs are required to limit spreading of these clones.
(© 2024. The Author(s).)
Databáze: MEDLINE
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