Microwave extraction and molecular imprinted polymer isolation of bergenin applied to the dendrochronological chemical study of Peltophorum dubium.

Autor: Silva-Neto OC; Instituto de Química, Universidade Federal da Bahia Campus Ondina, Salvador, BA, 40170280, Brazil., Felix CSA; Instituto de Química, Universidade Federal da Bahia Campus Ondina, Salvador, BA, 40170280, Brazil., de Oliveira Aguiar L; Instituto de Química, Universidade Federal da Bahia Campus Ondina, Salvador, BA, 40170280, Brazil., Dos Santos MB; Instituto de Química, Universidade Federal da Bahia Campus Ondina, Salvador, BA, 40170280, Brazil., Cunha S; Instituto de Química, Universidade Federal da Bahia Campus Ondina, Salvador, BA, 40170280, Brazil., David JM; Instituto de Química, Universidade Federal da Bahia Campus Ondina, Salvador, BA, 40170280, Brazil. jmdavid@ufba.br.
Jazyk: angličtina
Zdroj: BMC chemistry [BMC Chem] 2024 Jan 13; Vol. 18 (1), pp. 13. Date of Electronic Publication: 2024 Jan 13.
DOI: 10.1186/s13065-024-01112-7
Abstrakt: This study describes methodologies for extracting and isolating bergenin, a C-glucoside of 4-O-methylgallic acid found in some plants and it presents various in vitro and in vivo biological activities. Bergenin was previously obtained from the Pelthophorum dubim (Fabaceae) roots with a good yield. Conventional chromatographic procedures of the CHCl 3 soluble fraction of the MeOH extract gave 3.62% of this glucoside. An HPLC/DAD method was also developed and validated for bergenin and its precursor, gallic acid quantifications. Microwave extractions with different solvents were tested to optimize the extraction of bergenin, varying the temperature and time. MAE (Microwave Assisted Extraction) was more efficient than conventional extraction procedures, giving a higher yield of bergenin per root mass (0.45% vs. 0.0839%). Molecularly imprinted polymer (MIP) and non-imprinted polymer (NIP) based on bergenin as the template molecule, methacrylic acid, and ethylene glycol dimethacrylate were synthesized and characterized by FTIR and SEM (Scanning Electron Microscopy). Bergenin adsorption experiments using MIP and NIP followed by molecular imprinted solid phase extraction (MISPE) showed that MIP had a higher selectivity for bergenin than NIP. A dendrochronological study using the proposed method for detection and quantification of gallic acid and bergenin in five P. dubium growth rings of a 31-year-old heartwood and in the phelloderm and barks indicated that bergenin was more abundant in the 11-14th growth rings of the heartwood and decreased from the heartwood to the barks.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje