Prevalence of SARS-CoV-2 variants in Prague wastewater determined by nanopore-based sequencing.

Autor: Dostálková A; Department of Biotechnology, University of Chemistry and Technology Prague, Czech Republic; National Institute of Virology and Bacteriology, University of Chemistry and Technology Prague, Czech Republic., Zdeňková K; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Czech Republic. Electronic address: zdenkovk@vscht.cz., Bartáčková J; Department of Water Technology and Environmental Engineering, University of Chemistry and Technology Prague, Czech Republic., Čermáková E; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Czech Republic., Kapisheva M; National Institute of Virology and Bacteriology, University of Chemistry and Technology Prague, Czech Republic., Lopez Marin MA; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Czech Republic., Kouba V; Department of Water Technology and Environmental Engineering, University of Chemistry and Technology Prague, Czech Republic., Sýkora P; PVK a.s., Prague Water Supply and Sewerage Company, Czech Republic., Chmel M; Department of Infectious Diseases, First Faculty of Medicine, Charles University and Military University Hospital Prague, Prague, Czech Republic; Military Health Institute, Military Medical Agency, Czech Republic., Bartoš O; Military Health Institute, Military Medical Agency, Czech Republic., Dresler J; Military Health Institute, Military Medical Agency, Czech Republic., Demnerová K; Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Czech Republic., Rumlová M; Department of Biotechnology, University of Chemistry and Technology Prague, Czech Republic; National Institute of Virology and Bacteriology, University of Chemistry and Technology Prague, Czech Republic., Bartáček J; Department of Water Technology and Environmental Engineering, University of Chemistry and Technology Prague, Czech Republic.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2024 Mar; Vol. 351, pp. 141162. Date of Electronic Publication: 2024 Jan 11.
DOI: 10.1016/j.chemosphere.2024.141162
Abstrakt: The early detection of upcoming disease outbreaks is essential to avoid both health and economic damage. The last four years of COVID-19 pandemic have proven wastewater-based epidemiology is a reliable system for monitoring the spread of SARS-CoV-2, a causative agent of COVID-19, in an urban population. As this monitoring enables the identification of the prevalence of spreading variants of SARS-CoV-2, it could provide a critical tool in the fight against this viral disease. In this study, we evaluated the presence of variants and subvariants of SARS-CoV-2 in Prague wastewater using nanopore-based sequencing. During August 2021, the data clearly showed that the number of identified SARS-CoV-2 RNA copies increased in the wastewater earlier than in clinical samples indicating the upcoming wave of the Delta variant. New SARS-CoV-2 variants consistently prevailed in wastewater samples around a month after they already prevailed in clinical samples. We also analyzed wastewater samples from smaller sub-sewersheds of Prague and detected significant differences in SARS-CoV-2 lineage progression dynamics among individual localities studied, e.g., suggesting faster prevalence of new variants among the sites with highest population density and mobility.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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