Microplastics induced apoptosis in macrophages by promoting ROS generation and altering metabolic profiles.

Autor: Wang L; Department of Pathogen Biology, School of Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, PR China; Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu, PR China., Pei W; Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu, PR China., Li J; Department of Pathogen Biology, School of Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, PR China., Feng Y; Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu, PR China., Gao X; Department of Public Health, School of Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu, PR China., Jiang P; Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu, PR China., Wu Q; Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu, PR China., Li L; Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu, PR China. Electronic address: lilei@njmu.edu.cn.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2024 Feb; Vol. 271, pp. 115970. Date of Electronic Publication: 2024 Jan 13.
DOI: 10.1016/j.ecoenv.2024.115970
Abstrakt: The ubiquitous presence of Microplastics (MPs) in various environments documented in recent years has recently raised significant concerns about their toxic effects. While macrophages serve as the first line of defense against toxic substances and pathogens, the impact and mechanisms of microplastics on these immune cells remain unclear. This study aims to explore whether MPs induce macrophage apoptosis through the promotion of reactive oxygen species (ROS) generation and alterations in metabolic profiles. The viability of RAW264.7 cells decreased as the concentration of 0.5 µm or 5 µm MPs ranged from 0.2 to 1.5 mg/mL, with a more pronounced effect observed in the 0.5 µm MPs group. Zebrafish exposed to 0.5 µm or 5 µm MPs at a concentration of 0.5 mg/mL exhibited decreased macrophage abundance and increased apoptosis, accompanied by alterations in the expression of inflammatory and apoptosis-related genes. While 0.5 µm MPs were observed to enter macrophages, 5 µm MPs only adhered to the cell membrane surface. Both particle sizes induced ROS generation and disrupted cellular metabolism in RAW264.7 cells. Notably, macrophages exhibited a more pronounced response to 0.5 µm MPs, characterized by heightened ROS generation, increased secretion of pro-inflammatory mediators, and a significant decrease in sphingolipid metabolism. These findings suggest that the adverse effects on macrophages are greater with 0.5 µm MPs compared to 5 µm MPs, possibly attributed to particle size effects. This study contributes additional evidence on the impact of MPs on human immune cells.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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