Airway disease decreases the therapeutic potential of epithelial stem cells.

Autor: Zhang L; Department of Otolaryngology-Head and Neck Surgery, The Ohio State Wexner Medical Center, Columbus, OH, USA.; The Ohio State University College of Medicine, Columbus, OH, USA., Kelly N; Department of Otolaryngology, Nationwide Children's Hospital, 555 S. 18th St, Suite 2A, Columbus, OH, 43205, USA., Shontz KM; Center for Regenerative Medicine, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, OH, USA., Hill CL; Center for Perinatal Research, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, OH, USA., Stack JT; Center for Regenerative Medicine, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, OH, USA.; Center for Perinatal Research, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, OH, USA., Calyeca J; Department of Otolaryngology, Nationwide Children's Hospital, 555 S. 18th St, Suite 2A, Columbus, OH, 43205, USA.; Center for Regenerative Medicine, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, OH, USA., Matrka L; Department of Otolaryngology-Head and Neck Surgery, The Ohio State Wexner Medical Center, Columbus, OH, USA.; The Ohio State University College of Medicine, Columbus, OH, USA., Miller A; Comprehensive Center for Bronchopulmonary Dysplasia, Department of Pediatrics, Division of Neonatology, Nationwide Children's Hospital, Columbus, OH, USA., Reynolds SD; Center for Perinatal Research, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, OH, USA., Chiang T; Department of Otolaryngology-Head and Neck Surgery, The Ohio State Wexner Medical Center, Columbus, OH, USA. tendy.chiang@nationwidechildrens.org.; The Ohio State University College of Medicine, Columbus, OH, USA. tendy.chiang@nationwidechildrens.org.; Department of Otolaryngology, Nationwide Children's Hospital, 555 S. 18th St, Suite 2A, Columbus, OH, 43205, USA. tendy.chiang@nationwidechildrens.org.; Center for Regenerative Medicine, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, OH, USA. tendy.chiang@nationwidechildrens.org.
Jazyk: angličtina
Zdroj: Respiratory research [Respir Res] 2024 Jan 12; Vol. 25 (1), pp. 28. Date of Electronic Publication: 2024 Jan 12.
DOI: 10.1186/s12931-024-02667-8
Abstrakt: Backgorund: Tissue-engineered tracheal grafts (TETG) can be recellularized by the host or pre-seeded with host-derived cells. However, the impact of airway disease on the recellularization process is unknown.
Methods: In this study, we determined if airway disease alters the regenerative potential of the human tracheobronchial epithelium (hTBE) obtained by brushing the tracheal mucosa during clinically-indicated bronchoscopy from 48 pediatric and six adult patients.
Results: Our findings revealed that basal cell recovery and frequency did not vary by age or region. At passage 1, all samples produced enough cells to cellularize a 3.5 by 0.5 cm 2 graft scaffold at low cell density (~ 7000 cells/cm 2 ), and 43.75% could cellularize a scaffold at high cell density (~ 100,000 cells/cm 2 ). At passage 2, all samples produced the number of cells required for both recellularization models. Further evaluation revealed that six pediatric samples (11%) and three (50%) adult samples contained basal cells with a squamous basal phenotype. These cells did not form a polarized epithelium or produce differentiated secretory or ciliated cells. In the pediatric population, the squamous basal cell phenotype was associated with degree of prematurity (< 28 weeks, 64% vs. 13%, p = 0.02), significant pulmonary history (83% vs. 34%, p = 0.02), specifically with bronchopulmonary dysplasia (67% vs. 19%, p = 0.01), and patients who underwent previous tracheostomy (67% vs. 23%, p = 0.03).
Conclusions: In summary, screening high-risk pediatric or adult population based on clinical risk factors and laboratory findings could define appropriate candidates for airway reconstruction with tracheal scaffolds.
Level of Evidence: Level III Cohort study.
(© 2024. The Author(s).)
Databáze: MEDLINE
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