Metagenomic analysis of the ocular toxoplasmosis in children uveitis from Fayoum governorate, Egypt.

Autor: Elmallawany MA; Medical Parasitology Department, Faculty of Medicine Kasr Al-Ainy, Cairo University, 11796 Giza, Egypt., Abdel-Aal AA; Medical Parasitology Department, Faculty of Medicine Kasr Al-Ainy, Cairo University, 11796 Giza, Egypt; Postgraduate Department, Armed Forces College of Medicine (AFCM), 11774 Cairo, Egypt., Abu Eleinen KG; Ophthalmology Department, Faculty of Medicine Kasr Al-Ainy, Cairo University, 11796 Giza, Egypt., Nadar AH; Fayoum Ophthalmic Hospital, 63514 Fayoum, Egypt., El-Adawy AI; Medical Parasitology Department, Faculty of Medicine Kasr Al-Ainy, Cairo University, 11796 Giza, Egypt; Medical Parasitology Department, Armed Forces College of Medicine (AFCM), 11774 Cairo, Egypt., El-Dardiry MA; Medical Parasitology Department, Faculty of Medicine, Fayoum University, 63511 Fayoum, Egypt. Electronic address: marwaahmed2008@outlook.com., Abddel-Hafez YN; Medical Parasitology Department, Faculty of Medicine, Fayoum University, 63511 Fayoum, Egypt., Kotb AA; Ophthalmology Department, Faculty of Medicine, Fayoum University, 63511 Fayoum, Egypt., Saif ATS; Ophthalmology Department, Faculty of Medicine, Fayoum University, 63511 Fayoum, Egypt., Shaheen HAA; Medical Parasitology Department, Faculty of Medicine, Girl's Campus, Al-Azhar University, 11682 Cairo, Egypt., Sayed A; Basic Research Unit, Genomics/Epigenomics Program, Children's Cancer Hospital 57357, 11562 Cairo, Egypt., Samir O; Basic Research Unit, Genomics/Epigenomics Program, Children's Cancer Hospital 57357, 11562 Cairo, Egypt., Alatyar AA; Armed Forces College of Medicine (AFCM), 11774 Cairo, Egypt., Sheble MA; Armed Forces College of Medicine (AFCM), 11774 Cairo, Egypt., Elnakib M; Medical Microbiology and Immunology Department, Military Medical Academy, Cairo 11711, Egypt., Badr MS; Department of Molecular Biology and Bioinformatics, Medical Research Center, Faculty of Medicine, Ain Shams University, 11566 Cairo, Egypt., Nahnoush RK; Medical Parasitology Department, Faculty of Medicine Kasr Al-Ainy, Cairo University, 11796 Giza, Egypt; Medical Parasitology Department, Armed Forces College of Medicine (AFCM), 11774 Cairo, Egypt.
Jazyk: angličtina
Zdroj: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2024 Mar; Vol. 118, pp. 105551. Date of Electronic Publication: 2024 Jan 11.
DOI: 10.1016/j.meegid.2024.105551
Abstrakt: Granulomatous anterior uveitis with single or numerous gelatinous nodules was found in children living in rural Egypt. All ocular diseases were originally thought to be water-born and related to digenic flukes. The current study sought to learn more about the causes of anterior granulomatous uveitis in Egyptian youngsters who used to swim in rural water canals. 50 children with eye lesions that had not responded to medical treatment were recruited. Four samples were surgically extracted and examined using real-time PCR, transmission electron microscopy (TEM), and shotgun metagenomic sequencing (SMS). Toxoplasma gondii was detected free within the syncytium's distal section, while the proximal part exhibited active synthesis of a presumably extra-polymeric material, possibly released by the microbial population. Toxoplasma gondii was found in 30 samples. Serologically, distinct anti-Toxoplasma antibodies were not found in 91.6% of patients. SMS showed that the T. gondii ME 49 strain had the greatest percentage (29-25%) in all samples within an Acinetobacter-containing microbial community. These findings suggested that these bacteria entered the body via the exterior route rather than the circulatory route. The lack of genetic evidence for subsequent parasite stages invalidates the prior findings about the assumed trematode stage.
Competing Interests: Declaration of competing interest None.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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