Analysis of the molecular mechanisms regulating how ZmEREB24 improves drought tolerance in maize (Zea mays) seedlings.

Autor: Ren Z; College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China., Fu J; Henna Technology Innovation Centre of Wheat, Henan Agricultural University, Zhengzhou, 450046, China., Abou-Elwafa SF; Agronomy Department, Faculty of Agriculture, Assiut University, Assiut, Egypt., Ku L; College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China., Xie X; Henna Technology Innovation Centre of Wheat, Henan Agricultural University, Zhengzhou, 450046, China., Liu Z; College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China., Shao J; Henna Technology Innovation Centre of Wheat, Henan Agricultural University, Zhengzhou, 450046, China., Wen P; College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China., Al Aboud NM; Department of Biology, Faculty of Applied Sciences, Umm Al-Qura University, Makkah, 21955, Saudi Arabia., Su H; College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address: alice_suhui@126.com., Wang T; College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address: wtcwrn@126.com., Wei L; Henna Technology Innovation Centre of Wheat, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address: weili-wtc@126.com.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Feb; Vol. 207, pp. 108292. Date of Electronic Publication: 2023 Dec 22.
DOI: 10.1016/j.plaphy.2023.108292
Abstrakt: Drought stress is one of the most limiting factors of maize productivity and can lead to a sharp reduction in the total biomass when it occurs at the seedling stage. Improving drought tolerance at the seedling stage is of great importance for maize breeding. The AP2/ERF transcription factor family plays a critical role in plant response to abiotic stresses. Here, we used a preliminary previously-generated ranscriptomic dataset to identify a highly drought-stress-responsive AP2 gene, i.e., ZmEREB24. Compared to the wild type, the overexpression of ZmEREB24 in maize significantly promotes drought tolerance of transgenic plants at the seedling stage. CRISPR/Cas9-based ZmEREB24-knockout mutants showed a drought-sensitive phenotype. RNA-seq analysis and EMSA assay revealed AATGG.CT and GTG.T.GCC motifs as the main binding sites of ZmEREB24 to the promoters of downstream target genes. DAP-seq identified four novel target genes involved in proline and sugar metabolism and hormone signal transduction of ZmEREB24. Our data indicate that ZmEREB24 plays important biological functions in regulating drought tolerance by binding to the promoters of drought stress genes and modulating their expression. The results further suggest a role of ZmEREB24 in regulating drought adaptation in maize, indicating its potential importance for employing molecular breeding in the development of high-yield drought-tolerant maize cultivars.
Competing Interests: Declaration of competing interest We have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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