Ecological prevalence, genetic diversity, and multidrug resistance of Salmonella enteritidis recovered from broiler and layer chicken farms.

Autor:	Elsayed MM; Department of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., El-Basrey YFH; Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt., El-Baz AH; Department of Food Hygiene, Safety and Technology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., Dowidar HA; Department of Medical Laboratory, Higher Institute of Technology for Applied Health Science, Badr Institute for Science and Technology, Cairo, Egypt., Shami A; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi Arabia., Al-Saeed FA; Department of Biology, College of Science, King Khalid University, Abha 61421, Saudi Arabia., Alsamghan A; Family and Community Medicine Department, College of Medicine, King Khalid University, Abha 61413, Saudi Arabia., Salem HM; Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt., Alhazmi WA; Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia., El-Tarabily KA; Department of Biology, College of Science, United Arab Emirates University, Al Ain 15551, United Arab Emirates. Electronic address: ktarabily@uaeu.ac.ae., Khedr MHE; Department of Veterinary Public Health, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
Jazyk:	angličtina
Zdroj:	Poultry science [Poult Sci] 2024 Feb; Vol. 103 (2), pp. 103320. Date of Electronic Publication: 2023 Nov 25.
DOI:	10.1016/j.psj.2023.103320
Abstrakt:	Salmonella is a significant foodborne pathogen that has a significant impact on public health, and different strains of multidrug resistance (MDR) have been identified in this genus. This study used a combination of phenotypic and genotypic approaches to identify distinct Salmonella species collected from poultry broiler and layer farms, and antibiotic sensitivity testing was performed on these species. A total of 56 Salmonella isolates were serotyped, and phenotypic antibiotic resistance was determined for each strain. The enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) method was also used to provide a genotypic description, from which a dendrogram was constructed and the most likely phylogenetic relationships were applied. Salmonella isolates were detected in 20 (17%) out of 117 samples collected from small-scale broiler flocks. Salmonella isolates were classified as MDR strains after showing tolerance to 4 antibiotics, but no resistance to cloxacillin, streptomycin, vancomycin, or netilmicin was observed. From a genotypic perspective, these strains lack dfrD, parC, and blasfo-1 resistant genes, while harboring blactx-M, blaDHA-L, qnrA, qnrB, qnrS, gyrA, ermA, ermB, ermC, ermTR, mefA, msrA, tet A, tet B, tet L, tet M resistance genes. The genotyping results obtained with ERIC-PCR allowed isolates to be classified based on the source of recovery. It was determined that Salmonella strains displayed MDR, and many genes associated with them. Additionally, the ERIC-PCR procedure aided in the generation of clusters with biological significance. Extensive research on Salmonella serotypes is warranted, along with the implementation of long-term surveillance programs to monitor MDR Salmonella serotypes in avian-derived foods. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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