Effects of 625 nm light-emitting diode irradiation on preventing ER stress-induced apoptosis via GSK-3β phosphorylation in MC3T3-E1.

Autor: Fu W; Department of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju, Korea., Liu G; Department of Endodontics, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China.; Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea., Kim SH; Department of Oral Anatomy, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea., Kim B; Department of Oral Medicine, School of Dentistry, Chonnam National University, Gwangju, Korea., Kim OS; Department of Periodontology, School of Dentistry, Chonnam National University, Gwangju, Korea., Ma G; Department of Oral and Maxillofacial Surgery, School of Stomatology, Dalian Medical University, Dalian, China., Yang Y; Department of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju, Korea.; Dental Implant Center, School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, China., Liu D; Department of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju, Korea., Zhu S; Department of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju, Korea., Kang JS; Department of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju, Korea., Kim O; Department of Oral Pathology, School of Dentistry, Chonnam National University, Gwangju, Korea.
Jazyk: angličtina
Zdroj: Photochemistry and photobiology [Photochem Photobiol] 2024 Sep-Oct; Vol. 100 (5), pp. 1408-1418. Date of Electronic Publication: 2024 Jan 12.
DOI: 10.1111/php.13906
Abstrakt: Prolonged endoplasmic reticulum (ER) stress contributes to cell apoptosis and interferes with bone homeostasis. Although photobiomodulation (PBM) might be used for ER stress-induced diseases, the role of PBM in relieving cell apoptosis remains unknown. During ER stress, glycogen synthase kinase-3β (GSK-3β) is critical; however, its functions in PBM remain uncertain. Thus, this study aimed to investigate the role of GSK-3β in 625 nm light-emitting diode irradiation (LEDI) relieving tunicamycin (TM)-induced apoptosis. Based on the results, pre-625 nm LEDI (Pre-IR) phosphorylated GSK-3β via ROS production. Compared with the TM group, Pre-IR + TM group reduced the phosphorylation of the α-subunit of eukaryotic translation initiation factor 2 (eIF-2α) and B-cell lymphoma protein 2 (Bcl-2)-associated X (Bax)/Bcl-2 ratio through regulating GSK-3β. Furthermore, a similar tendency was observed between Pre-IR + TM and Pre-LiCl+TM groups in preventing TM-induced early and late apoptosis. In summary, this study suggests that the Pre-IR treatment in TM-induced ER stress is beneficial for preventing cell apoptosis via GSK-3β phosphorylation.
(© 2024 American Society for Photobiology.)
Databáze: MEDLINE
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