A cell line derived from heart of rainbow trout is refractory to Tilapia lake virus.

Autor: Pande A; ICAR-Directorate of Coldwater Fisheries Research, Bhimtal, Uttarakhand, India., Thakuria D; ICAR-Directorate of Coldwater Fisheries Research, Bhimtal, Uttarakhand, India., Kushwaha B; ICAR-National Bureau of Fish Genetic Resources, Lucknow, Uttar Pradesh, India., Kumar R; ICAR-National Bureau of Fish Genetic Resources, Lucknow, Uttar Pradesh, India., S M; ICAR-National Bureau of Fish Genetic Resources, Lucknow, Uttar Pradesh, India., Rastogi A; ICAR-National Bureau of Fish Genetic Resources, Lucknow, Uttar Pradesh, India., Sood N; ICAR-National Bureau of Fish Genetic Resources, Lucknow, Uttar Pradesh, India.
Jazyk: angličtina
Zdroj: Cell biology international [Cell Biol Int] 2024 Mar; Vol. 48 (3), pp. 347-357. Date of Electronic Publication: 2024 Jan 11.
DOI: 10.1002/cbin.12125
Abstrakt: Cell lines are important in vitro models to answer biological mechanisms with less genetic variations. The present study was attempted to develop a cell line from rainbow trout, where we obtained a cell line from the heart, named "RBT-H." The cell line was authenticated using karyotyping and cytochrome c oxidase subunit I (COI) gene sequencing. The karyotype demonstrated diploid chromosome number (2n) as 62 and the sequence of partial COI gene was 99.84% similar to rainbow trout COI data set, both suggesting the origin of RBT-H from the rainbow trout. The heart cell line was mycoplasma-free and found to be refractory to infection with the Tilapia lake virus. The RBT-H cell line is deposited in the National Repository of Fish Cell Line (NRFC) at ICAR-NBFGR, Lucknow, India, with Accession no. NRFC0075 for maintenance and distribution to researchers on request for R&D.
(© 2024 International Federation of Cell Biology.)
Databáze: MEDLINE
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