Hair Follicle Damage after 100 mGy Low-Dose Fractionated X-Ray Irradiation and the Protective Effects of TEMPOL, a Stable Nitroxide Radical, against Radiation.

Autor:	Kawabata Y; Department of Oncology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8544, Japan., Fukushige T; Department of Dermatology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8544, Japan., Indo HP; Department of Oncology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8544, Japan.; Amanogawa Galaxy Astronomy Research Center, Kagoshima University Graduate School of and Engineering, Kagoshima 890-0065, Japan., Matsumoto KI; Quantitative RedOx Sensing Group, Department of Radiation Regulatory Science Research, National Institute of Radiological Sciences, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan., Ueno M; Quantitative RedOx Sensing Group, Department of Radiation Regulatory Science Research, National Institute of Radiological Sciences, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan., Nakanishi I; Quantum RedOx Chemistry Team, Institute for Quantum Life Science (iQLS), Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology (QST), 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan., Chatatikun M; Biomedical Sciences, School of Allied Health Sciences, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand.; Center of Excellence Research for Melioidosis and Microorganisms, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand., Klangbud WK; Biomedical Sciences, School of Allied Health Sciences, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand.; Center of Excellence Research for Melioidosis and Microorganisms, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand., Surinkaew S; Biomedical Sciences, School of Allied Health Sciences, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand.; Research Excellence Center for Innovation and Health Products (RECIHP), School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand., Tangpong J; Biomedical Sciences, School of Allied Health Sciences, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand.; Research Excellence Center for Innovation and Health Products (RECIHP), School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand., Kanekura T; Department of Dermatology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8544, Japan., Majima HJ; Department of Oncology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8544, Japan.; Amanogawa Galaxy Astronomy Research Center, Kagoshima University Graduate School of and Engineering, Kagoshima 890-0065, Japan.; Center of Excellence Research for Melioidosis and Microorganisms, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand.; Research Excellence Center for Innovation and Health Products (RECIHP), School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand.; Department of Space Environmental Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8544, Japan.
Jazyk:	angličtina
Zdroj:	Radiation research [Radiat Res] 2024 Feb 01; Vol. 201 (2), pp. 115-125.
DOI:	10.1667/RADE-23-00167.1
Abstrakt:	The effects of long-term low-dose X-ray irradiation on the outer root sheath (ORS) cells of C3H/He mice were investigated. Mice were irradiated with a regime of 100 mGy/day, 5 days/week, for 12 weeks (Group X) and the results obtained were compared to those in a non-irradiated control (Group C). Potential protection against ORS cells damage induced by this exposure was investigated by adding the stable nitroxide radical 4-hydroxyl-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPOL) at 1 mM to the drinking water of mice (Group X + TEMPOL). The results obtained were compared with Group C and a non-irradiated group treated with TEMPOL (Group C + TEMPOL). After fractionated X-ray irradiation, skin was removed and ORS cells were examined by hematoxylin and eosin staining and electron microscopy for an abnormal nuclear morphology and nuclear condensation changes. Fractionated X-irradiated mice had an increased number of ORS cells with an abnormal nuclear morphology as well as nuclear condensation changes. Sections were also immunohistochemically examined for the presence of TdT-mediated dUTP nick-end labeling (TUNEL), 8-hydroxy-2'-deoxyguanosine (8-OHdG), 4-hydroxynonenal (4-HNE), vascular endothelial growth factor (VEGF), nitrotyrosine, heme oxygenase 1 (HO-1), and protein gene product 9.5 (PGP 9.5). Significant increases were observed in TUNEL, 8-OHdG, and 4-HNE levels in ORS cells from mice in Group X. Electron microscopy also showed irregular shrunken ORS cells in Group X. These changes were prevented by the presence of TEMPOL in the drinking water of the irradiated mice. TEMPOL alone had no significant effects. These results suggest that fractionated doses of radiation induced oxidative damage in ORS cells; however, TEMPOL provided protection against this damage, possibly as a result of the rapid reaction of this nitroxide radical with the reactive oxidants generated by fractionated X-ray irradiation. (© 2024 by Radiation Research Society.)
Databáze:	MEDLINE
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