Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass.

Autor: Žižić M; Department of Life Sciences, University of Belgrade, Institute for Multidisciplinary Research, Kneza Višeslava 1, 11030 Belgrade, Serbia; Elettra -Sincrotrone Trieste, Strada Statale 14 - km 163, 5 in AREA Science Park, Trieste, Italy. Electronic address: mzizic@imsi.rs., Atlagić K; Department of Physiology and Biophysics, University of Belgrade, Faculty of Biology, Studentski trg 16, 11158 Belgrade, Serbia., Karaman M; Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 2, 21000 Novi Sad, Serbia., Živić M; Department of Physiology and Biophysics, University of Belgrade, Faculty of Biology, Studentski trg 16, 11158 Belgrade, Serbia., Stanić M; Department of Life Sciences, University of Belgrade, Institute for Multidisciplinary Research, Kneza Višeslava 1, 11030 Belgrade, Serbia., Maksimović V; Department of Life Sciences, University of Belgrade, Institute for Multidisciplinary Research, Kneza Višeslava 1, 11030 Belgrade, Serbia., Zakrzewska J; Institute of General and Physical Chemistry, Studentski trg 12, 11158 Belgrade, Serbia.
Jazyk: angličtina
Zdroj: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) [J Trace Elem Med Biol] 2024 May; Vol. 83, pp. 127381. Date of Electronic Publication: 2024 Jan 04.
DOI: 10.1016/j.jtemb.2024.127381
Abstrakt: Background: Fungi absorb and solubilize a broad spectrum of heavy metals such as vanadium (V), which makes them a main route of its entry into the biosphere. V as vanadate (V 5+ ) is a potential medical agent due to its many metabolic actions such as interaction with phosphates in the cell, and especially its insulin-mimetic activity. Antidiabetic activity of V-enriched fungi has been studied in recent years, but the biological and chemical bases of vanadium action and status in fungi in general are poorly understood, with almost no information on edible fungi.
Methods: This manuscript gives a deeper insight into the interaction of V 5+ with Coprinellus truncorum, an edible autochthonous species widely distributed in Europe and North America. Vanadium uptake and accumulation as V 5+ was studied by 51 V NMR, while the reducing abilities of the mycelium were determined by EPR. 31 P NMR was used to determine its effects on the metabolism of phosphate compounds, with particular focus on phosphate sugars identified using HPLC.
Results: Vanadate enters the mycelium in monomeric form and shows no immediate detrimental effects on intracellular pH or polyphosphate (PPc) levels, even when applied at physiologically high concentrations (20 mM Na 3 VO 4 ). Once absorbed, it is partially reduced to less toxic vanadyl (V 4+ ) with notable unreduced portion, which leads to a large increase in phosphorylated sugar levels, especially glucose-1-phosphate (G1P) and fructose-6-phosphate (F6P).
Conclusions: Preservation of pH and especially PPc reflects maintenance of the energy status of the mycelium, i.e., its tolerance to high V 5+ concentrations. Rise in G1P and F6P levels implies that the main targets of V 5+ are most likely phosphoglucomutase and phosphoglucokinase(s), enzymes involved in early stages of G6P transformation in glycolysis and glycogen metabolism. This study recommends C. truncorum for further investigation as a potential antidiabetic agent.
Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest.
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Databáze: MEDLINE
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