| Autor: |
Zhang W; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China. yunyunquan@163.com., Wu B; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China. yunyunquan@163.com., Liang M; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China. yunyunquan@163.com., Zhang M; The Affiliated Xiangshan Hospital of Wenzhou Medical University: Xiangshan First People's Hospital Medical and Health Group, Xiangshan 315700, China. shenren34127@163.com., Hu Y; The Affiliated Xiangshan Hospital of Wenzhou Medical University: Xiangshan First People's Hospital Medical and Health Group, Xiangshan 315700, China. shenren34127@163.com., Huang ZS; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China. yunyunquan@163.com., Ye X; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China. yunyunquan@163.com., Du B; The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325027, China. 443723729@qq.com., Quan YY; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China. yunyunquan@163.com., Jiang Y; The Affiliated Xiangshan Hospital of Wenzhou Medical University: Xiangshan First People's Hospital Medical and Health Group, Xiangshan 315700, China. shenren34127@163.com. |
| Abstrakt: |
Cysteine (Cys) and homocysteine (Hcy) are important biothiols in living organisms. They play important roles in a variety of physiological and pathological processes. Therefore, it is very important to design an optical probe for the selective detection of Cys/Hcy. Herein, we report the design and synthesis of a fluorescent probe NBD-B-T based on a boron-dipyrromethene (BODIPY) structure, which showed an excellent lysosome targeting ability and an outstanding Cys/Hcy detection capacity. For NBD-B-T, the sensing group 7-nitro-2,1,3-benzoxadiazole (NBD) and the lysosomal targeting group morpholine were introduced. The results show that the NBD-B-T probe can detect Cys/Hcy with fluorescence emission turn-on performance. The low detection limits of this probe are about 76.0 nM for Hcy and 97.6 nM for Cys, respectively. The NBD-B-T probe has a low detection limit, high stability, and excellent selectivity and sensitivity. More importantly, the NBD-B-T can target lysosome, and simultaneously detect the Cys/Hcy in living cells. |
