Morphological, Biochemical, and Molecular Diversity Assessment of Egyptian Bottle Gourd Cultivars.

Autor: Ibrahim EA; Department of Cross-Pollinated Vegetable Research, Horticulture Research Institute, Agricultural Research Centre, Giza 12619, Egypt., Alhaithloul HAS; Biology Department, College of Science, Jouf University, Sakaka 2014, Aljouf, Saudi Arabia., Shamseldin SAM; Botany Department, Women's College for Arts, Science and Education, Ain Shams University, Cairo 11566, Egypt., Awaly SBH; Genetics Department, Faculty of Agriculture, Cairo University, Giza, Egypt., El-Latif Hesham A; Genetics Department, Faculty of Agriculture, Beni-Suef University, Beni Suef 62521, Egypt., Abdelkader MFM; Department of Plant Production, College of Food and Agriculture, King Saud University, Riyadh 12372, Saudi Arabia., Alqahtani MM; Department of Biological Sciences, Faculty of Science and Humanities, Shaqra University, P.O. Box 1040, Ad-Dawadimi, Shaqra 11911, Saudi Arabia., Alzuaibr FM; Biology Department, College of Science, Tabuk University, Tabuk 71491, Saudi Arabia., Alasmari A; Biology Department, College of Science, Tabuk University, Tabuk 71491, Saudi Arabia., Sukar NA; Agricultural Botany (Genetics) Department, Faculty of Agriculture (Girls), Al-Azhar University, Cairo, Egypt., Diyasty MZ; Genetic Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt., Abdein MA; Seeds Development Department, El-Nada Misr Scientific Research and Development Projects, Turrell, Mansoura 35511, Egypt.
Jazyk: angličtina
Zdroj: Genetics research [Genet Res (Camb)] 2024 Jan 03; Vol. 2024, pp. 4182158. Date of Electronic Publication: 2024 Jan 03 (Print Publication: 2024).
DOI: 10.1155/2024/4182158
Abstrakt: The genetic variability and relationships between ten bottle gourd cultivars were evaluated based on morphological, biochemical, and molecular parameters. The results displayed high variability among selected cultivars in terms of photosynthetic pigments, total free amino acids, total phenol content, isozymes pattern, and protein electrophoresis. Furthermore, differences in molecular markers were revealed by the SCoT technique. The peroxidase (POD) and polyphenyl oxidase (PPO) isozymes patterns did not detect significant differences in bands among cultivars. The protein patterns revealed seventeen bands ranging from 126 to 9 kDa and five polymorphic bands representing 29.41%. On the other hand, eight SCoT primers were used to evaluate the genetic variability and relationships between the ten Egyptian bottle gourd cultivars. The results of SCoT analysis detected 44 amplicons with 50% polymorphism. In addition, the results of the phylogenetic tree that is constructed based on the similarity coefficient revealed by SCoT analysis confirm the results of biochemical analysis indicating a genetic relationship between the most efficient bottle gourd cultivars (S1 and S2 cultivars). In addition, there is a genetic relationship among the less efficient bottle gourd cultivars (S4 and S5 cultivars). These results could be beneficial to distinguish among bottle gourd cultivars in the plant breeding programs.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2024 Ehab A. Ibrahim et al.)
Databáze: MEDLINE