Role of Arctiin in Fibrosis and Apoptosis in Experimentally Induced Hepatocellular Carcinoma in Rats.

Autor:	Alshehri SA; Pharmacy, University of Tabuk Faculty of Pharmacy, Tabuk, SAU., Almarwani WA; Pharmacy, University of Tabuk Faculty of Pharmacy, Tabuk, SAU., Albalawi AZ; Pharmacy, University of Tabuk Faculty of Pharmacy, Tabuk, SAU., Al-Atwi SM; Pharmacy, University of Tabuk Faculty of Pharmacy, Tabuk, SAU., Aljohani KK; Pharmacy, University of Tabuk Faculty of Pharmacy, Tabuk, SAU., Alanazi AA; Pharmacy, King Salman Armed Forced Hospital, Tabuk, SAU., Ebrahim MA; Medical Oncology, Oncology Center, Mansoura University, Mansoura, EGY., Hassan HM; Pharmacology and Biochemistry, Delta University for Science and Technology, Gamasa, EGY., Al-Gayyar MM; Pharmaceutical Chemistry, University of Tabuk Faculty of Pharmacy, Tabuk, SAU.; Biochemistry, Mansoura University Faculty of Pharmacy, Mansoura, EGY.
Jazyk:	angličtina
Zdroj:	Cureus [Cureus] 2024 Jan 10; Vol. 16 (1), pp. e51997. Date of Electronic Publication: 2024 Jan 10 (Print Publication: 2024).
DOI:	10.7759/cureus.51997
Abstrakt:	Background and objectives Hepatocellular carcinoma (HCC) is a highly aggressive malignant tumor with a poor prognosis. It is currently the second most common cause of cancer-related mortality. Arctiin, a compound found in plants commonly used as a vegetable in Asian countries and as an ingredient in traditional European dishes, possesses various properties, including anti-proliferative, anti-senescence, anti-oxidative, anti-tumor, toxic, anti-adipogenic, and anti-bacterial effects. Our study aims to investigate the potential antitumor activity of arctiin against HCC in rats by inhibiting cell fibrosis and apoptosis. Methods Rats were induced with HCC by administering thioacetamide. Arctiin was orally administered to some rats twice a week for 16 weeks at a dose of 30 mg/kg. The liver impairment was evaluated by measuring serum α-fetoprotein (AFP) and examining liver sections stained with Masson trichrome or anti-hypoxia-induced factor-1α (HIF-1α) antibodies. The hepatic expression of messenger RNA and protein levels of HIF-1α, protein kinase C (PKC), extracellular signal-regulated kinase (ERK), β-catenin, and mothers against decapentaplegic homolog 4 (SMAD4) were analyzed. Results Our study demonstrated that arctiin can potentially increase the survival rate of rats. This is achieved through a reduction in serum AFP levels and hepatic nodules. We also observed that arctiin has the ability to inhibit the formation of fibrotic tissues and necrotic nodules in HCC rats. Additionally, arctiin can significantly decrease the expression of HIF-1α, PKC, ERK, β-catenin, and SMAD4. Conclusion Arctiin has demonstrated potential anti-tumor properties that could ameliorate HCC. Studies have shown that it may increase survival rates and reduce the number of tumors and AFP levels. Arctiin works by inhibiting HCC-induced hypoxia, thus blocking the expression of HIF-1α. It also helps to slow down tumor fibrosis by decreasing the expression of β-catenin and SMAD4. Furthermore, arctiin has been found to downregulate PKC and ERK, reducing hepatic tissue apoptosis. Competing Interests: The authors have declared that no competing interests exist. (Copyright © 2024, Alshehri et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu