Probing the Cellular Fate of the Protein Corona around Nanoparticles with Nanofocused X-ray Fluorescence Imaging.

Autor: Skiba M; Center for Hybrid Nanostructures, University of Hamburg, 22761 Hamburg, Germany.; The Hamburg Centre for Ultrafast Imaging, 22761 Hamburg, Germany., Guedes G; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), 20014 Donostia-San Sebastian, Spain., Karpov D; European Synchrotron Radiation Facility, 38000 Grenoble, France., Feliu N; Zentrum für Angewandte Nanotechnologie CAN, Fraunhofer-Institut für Angewandte Polymerforschung IAP, 20146 Hamburg, Germany., L Cortajarena A; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), 20014 Donostia-San Sebastian, Spain.; Ikerbasque, Basque Foundation for Science, 48009 Bilbao, Spain., Parak WJ; Center for Hybrid Nanostructures, University of Hamburg, 22761 Hamburg, Germany.; The Hamburg Centre for Ultrafast Imaging, 22761 Hamburg, Germany., Sanchez-Cano C; Ikerbasque, Basque Foundation for Science, 48009 Bilbao, Spain.; Donostia International Physics Center, 20018 Donostia-San Sebastian, Spain.; Polimero eta Material Aurreratuak: Fisika, Kimika eta Teknologia, Kimika Fakultatea, Euskal Herriko Unibertsitatea UPV/EHU, 20018 Donostia-San Sebastian, Spain.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2023 Dec 30; Vol. 25 (1). Date of Electronic Publication: 2023 Dec 30.
DOI: 10.3390/ijms25010528
Abstrakt: X-ray fluorescence imaging (XRF-imaging) with subcellular resolution is used to study the intracellular integrity of a protein corona that was pre-formed around gold nanoparticles (AuNP). Artificial proteins engineered to obtain Gd coordination for detection by XRF-imaging were used to form the corona. Indications about the degradation of this protein corona at a cellular and subcellular level can be observed by following the Au and Gd quantities in a time and spatial-dependent manner. The extended acquisition times necessary for capturing individual XRF-imaging cell images result in relatively small sample populations, stressing the need for faster image acquisition strategies in future XRF-imaging-based studies to deal with the inherent variability between cells. Still, results obtained reveal degradation of the protein corona during cellular trafficking, followed by differential cellular processing for AuNP and Gd-labelled proteins. Overall, this demonstrates that the dynamic degradation of the protein corona can be tracked by XRF-imaging to a certain degree.
Databáze: MEDLINE
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