| Autor: |
Kotlova ER; Komarov Botanical Institute, Russian Academy of Sciences, 197022 Saint-Petersburg, Russia., Senik SV; Komarov Botanical Institute, Russian Academy of Sciences, 197022 Saint-Petersburg, Russia., Pozhvanov GA; Komarov Botanical Institute, Russian Academy of Sciences, 197022 Saint-Petersburg, Russia.; Department of Botany and Ecology, Faculty of Biology, Herzen State Pedagogical University, 191186 Saint-Petersburg, Russia., Prokopiev IA; Komarov Botanical Institute, Russian Academy of Sciences, 197022 Saint-Petersburg, Russia., Boldyrev IA; Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119991 Moscow, Russia., Manzhieva BS; Komarov Botanical Institute, Russian Academy of Sciences, 197022 Saint-Petersburg, Russia., Amigud EY; Komarov Botanical Institute, Russian Academy of Sciences, 197022 Saint-Petersburg, Russia.; Department of Botany and Ecology, Faculty of Biology, Herzen State Pedagogical University, 191186 Saint-Petersburg, Russia., Puzanskiy RK; Komarov Botanical Institute, Russian Academy of Sciences, 197022 Saint-Petersburg, Russia., Khakulova AA; Chemical Analysis and Materials Research Core Facility Center, Reseach Park, Saint-Petersburg State University, 199034 Saint-Petersburg, Russia., Serebryakov EB; Chemical Analysis and Materials Research Core Facility Center, Reseach Park, Saint-Petersburg State University, 199034 Saint-Petersburg, Russia. |
| Abstrakt: |
Fungi and plants are not only capable of synthesizing the entire spectrum of lipids de novo but also possess a well-developed system that allows them to assimilate exogenous lipids. However, the role of structure in the ability of lipids to be absorbed and metabolized has not yet been characterized in detail. In the present work, targeted lipidomics of phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs), in parallel with morphological phenotyping, allowed for the identification of differences in the effects of PC molecular species introduced into the growth medium, in particular, typical bacterial saturated (14:0/14:0, 16:0/16:0), monounsaturated (16:0/18:1), and typical for fungi and plants polyunsaturated (16:0/18:2, 18:2/18:2) species, on Arabidopsis thaliana . For comparison, the influence of an artificially synthesized (1,2-di-(3-(3-hexylcyclopentyl)-propanoate)- sn -glycero-3-phosphatidylcholine, which is close in structure to archaeal lipids, was studied. The phenotype deviations stimulated by exogenous lipids included changes in the length and morphology of both the roots and leaves of seedlings. According to lipidomics data, the main trends in response to exogenous lipid exposure were an increase in the proportion of endogenic 18:1/18:1 PC and 18:1_18:2 PC molecular species and a decrease in the relative content of species with C18:3, such as 18:3/18:3 PC and/or 16:0_18:3 PC, 16:1_18:3 PE. The obtained data indicate that exogenous lipid molecules affect plant morphology not only due to their physical properties, which are manifested during incorporation into the membrane, but also due to the participation of exogenous lipid molecules in the metabolism of plant cells. The results obtained open the way to the use of PCs of different structures as cellular regulators. |
