| Autor: |
Jeong S; Department of New Biology, DGIST, Daegu 42988, Republic of Korea., Koh D; School of Undergraduate Studies, DGIST, Daegu 42988, Republic of Korea., Gwak E; Department of New Biology, DGIST, Daegu 42988, Republic of Korea., Srambickal CV; Exp. Biomol. Physics, Dept. Applied Physics, KTH-Royal Institute of Technology, 106 91 Stockholm, Sweden., Seo D; Department of Physics and Chemistry, DGIST, Daegu 42988, Republic of Korea., Widengren J; Exp. Biomol. Physics, Dept. Applied Physics, KTH-Royal Institute of Technology, 106 91 Stockholm, Sweden., Lee JC; Department of New Biology, DGIST, Daegu 42988, Republic of Korea.; New Biology Research Center, DGIST, Daegu 42988, Republic of Korea. |
| Abstrakt: |
Optical nanoscopy, also known as super-resolution optical microscopy, has provided scientists with the means to surpass the diffraction limit of light microscopy and attain new insights into nanoscopic structures and processes that were previously inaccessible. In recent decades, numerous studies have endeavored to enhance super-resolution microscopy in terms of its spatial (lateral) resolution, axial resolution, and temporal resolution. In this review, we discuss recent efforts to push the resolution limit of stimulated emission depletion (STED) optical nanoscopy across multiple dimensions, including lateral resolution, axial resolution, temporal resolution, and labeling precision. We introduce promising techniques and methodologies building on the STED concept that have emerged in the field, such as MINSTED, isotropic STED, and event-triggered STED, and evaluate their respective strengths and limitations. Moreover, we discuss trade-off relationships that exist in far-field optical microscopy and how they come about in STED optical nanoscopy. By examining the latest developments addressing these aspects, we aim to provide an updated overview of the current state of STED nanoscopy and its potential for future research. |
