| Autor: |
Mantovska DI; Department of Plant Physiology, Faculty of Biology, Sofia University 'St. Kliment Ohridski', 8 Dragan Tsankov Blvd., 1164 Sofia, Bulgaria., Zhiponova MK; Department of Plant Physiology, Faculty of Biology, Sofia University 'St. Kliment Ohridski', 8 Dragan Tsankov Blvd., 1164 Sofia, Bulgaria., Petrova D; Department of Plant Physiology, Faculty of Biology, Sofia University 'St. Kliment Ohridski', 8 Dragan Tsankov Blvd., 1164 Sofia, Bulgaria., Alipieva K; Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, bl. 9 Acad. Georgi Bonchev Str., 1113 Sofia, Bulgaria., Bonchev G; Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., Bl. 21, 1113 Sofia, Bulgaria., Boycheva I; Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., Bl. 21, 1113 Sofia, Bulgaria., Evstatieva Y; Department of Biotechnology, Faculty of Biology, Sofia University 'St. Kliment Ohridski', 8 Dragan Tsankov Blvd., 1164 Sofia, Bulgaria., Nikolova D; Department of Biotechnology, Faculty of Biology, Sofia University 'St. Kliment Ohridski', 8 Dragan Tsankov Blvd., 1164 Sofia, Bulgaria., Tsacheva I; Department of Biochemistry, Faculty of Biology, Sofia University 'St. Kliment Ohridski', 8 Dragan Tsankov Blvd., 1164 Sofia, Bulgaria., Simova S; Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, bl. 9 Acad. Georgi Bonchev Str., 1113 Sofia, Bulgaria., Yordanova ZP; Department of Plant Physiology, Faculty of Biology, Sofia University 'St. Kliment Ohridski', 8 Dragan Tsankov Blvd., 1164 Sofia, Bulgaria. |
| Abstrakt: |
Stachys scardica Griseb. is a Balkan endemic species listed in The Red Data Book of Bulgaria with the conservation status "endangered". Successful micropropagation was achieved on MS medium supplemented with 1.5 mg/L benzyladenine (BA), followed by a subsequent ex vitro adaptation in an experimental field resulting in 92% regenerated plants. Using nuclear magnetic resonance (NMR), phenylethanoid glycosides (verbascoside, leucosceptoside A), phenolic acids (chlorogenic acid), iridoids (allobetonicoside and 8-OAc-harpagide), and alkaloids (trigonelline) were identified, characteristic of plants belonging to the genus Stachys . High antioxidant and radical scavenging activities were observed in both in situ and ex vitro acclimated S. scardica plants, correlating with the reported high concentrations of total phenols and flavonoids in these variants. Ex vitro adapted plants also exhibited a well-defined anti-inflammatory potential, demonstrating high inhibitory activity against the complement system. Employing a disk diffusion method, a 100% inhibition effect was achieved compared to positive antibiotic controls against Staphylococcus epidermidis and Propionibacterium acnes , with moderate activity against Bacillus cereus . The induced in vitro and ex vitro model systems can enable the conservation of S. scardica in nature and offer future opportunities for the targeted biosynthesis of valuable secondary metabolites, with potential applications in the pharmaceutical and cosmetic industries. |
