Role of maraviroc and/or rapamycin in the liver of IL10 KO mice with frailty syndrome.

Autor: Pérez-Martínez L; Centro de Investigación Biomédica de La Rioja (CIBIR), Logroño, Spain., Romero L; Centro de Investigación Biomédica de La Rioja (CIBIR), Logroño, Spain., Verdugo-Sivianes EM; Instituto de Biomedicina de Sevilla, IBIS, Hospital Universitario Virgen del Rocío, Consejo Superior de Investigaciones Científicas, Universidad de Sevilla, Sevilla, Spain.; CIBERONC, Instituto de Salud Carlos III, Madrid, Spain., Muñoz-Galván S; Instituto de Biomedicina de Sevilla, IBIS, Hospital Universitario Virgen del Rocío, Consejo Superior de Investigaciones Científicas, Universidad de Sevilla, Sevilla, Spain.; CIBERONC, Instituto de Salud Carlos III, Madrid, Spain., Rubio-Mediavilla S; Servicio de Anatomía Patológica, Hospital San Pedro, Logroño, Spain., Amiama-Roig A; Centro de Investigación Biomédica de La Rioja (CIBIR), Logroño, Spain., Carnero A; Instituto de Biomedicina de Sevilla, IBIS, Hospital Universitario Virgen del Rocío, Consejo Superior de Investigaciones Científicas, Universidad de Sevilla, Sevilla, Spain.; CIBERONC, Instituto de Salud Carlos III, Madrid, Spain., Blanco JR; Centro de Investigación Biomédica de La Rioja (CIBIR), Logroño, Spain.; Servicio de Enfermedades Infecciosas, Hospital Universitario San Pedro, Logroño, Spain.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Jan 10; Vol. 19 (1), pp. e0286201. Date of Electronic Publication: 2024 Jan 10 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0286201
Abstrakt: Cellular senescence and low-grade inflammation favor the acceleration of aging. The liver is an essential metabolic organ because changes related to its function are related to age-related diseases. The objective of this study was to evaluate the effects of maraviroc (MVC) and/or rapamycin (RAPA) on liver tissue in an experimental model of frailty syndrome in mice, since MVC and RAPA are two molecules able to decrease CCR5 expression, which is overexpressed in patients with frailty. Methods: Eighty male homozygous IL10KO mice were randomly assigned to one of 4 groups (n = 20): i) IL10KO group; ii) MVC group, iii) RAPA group, and iv) MVC-RAPA group. Liver samples were analyzed. Gene expression quantification and western blotting were also performed. The proinflammatory cytokines IL-6 and IL-18 were decreased in MVC and MVC/RAPA groups, IL-12 was decreased in RAPA and MVC/RAPA groups and TNF-α was decreased in all therapeutic groups. P21 was decreased in RAPA and MVC/RAPA groups, Galactosidase beta-1, was also significantly reduced in all therapeutic groups, as were NF-kB1, NF-kB2 and STAT3. In all groups, mTOR and CCL5 were significantly reduced. CCR5 expression was decreased in the MVC and MVC/RAPA groups. Conclusion: MVC and RAPA may protect against some factors involved in liver aging. More studies will be necessary to verify their clinical applications.
Competing Interests: All authors have read the journal’s authorship agreement and policy on disclosure of potential conflicts of interest. Dra. L. Pérez-Martínez has received compensation for lectures from Abbvie, Bristol-Myers Squibb, Gilead Sciences, and Janssen. Dr. J. R. Blanco has carried out consulting work for Abbvie, Bristol-Myers Squibb, Gilead Sciences, Janssen, Merck, ViiV Healthcare and THERA technologies; has received compensation for lectures from Abbvie, Bristol-Myers Squibb, Gilead Sciences, Janssen, Merck, and ViiV Healthcare, as well as grants and payments for the development of educational presentations for Gilead Sciences, Bristol-Myers Squibb and ViiV Healthcare. The rest of the authors declare no conflict of interest. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
(Copyright: © 2024 Pérez-Martínez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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