The ergodicity question when imaging DNA conformation using liquid cell electron microscopy.

Autor:	Li JY; Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, National Biomedical Imaging Center, Key Laboratory of Polymer Chemistry & Physics of Ministry of Education, Peking University, Beijing 100871, China., Liu F; Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, National Biomedical Imaging Center, Key Laboratory of Polymer Chemistry & Physics of Ministry of Education, Peking University, Beijing 100871, China.; Beijing NMR Center, School of Life Science, Peking University, Beijing 100871, China., Xu J; Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, National Biomedical Imaging Center, Key Laboratory of Polymer Chemistry & Physics of Ministry of Education, Peking University, Beijing 100871, China.; Beijing NMR Center, School of Life Science, Peking University, Beijing 100871, China., Kim YJ; Department of Chemistry, Ulsan National Institute of Science and Technology, Ulsan 44919, South Korea.; Center for Soft and Living Matter, Institute for Basic Science, Ulsan 44919, South Korea., Kwon OH; Department of Chemistry, Ulsan National Institute of Science and Technology, Ulsan 44919, South Korea.; Center for Soft and Living Matter, Institute for Basic Science, Ulsan 44919, South Korea., Xia B; Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, National Biomedical Imaging Center, Key Laboratory of Polymer Chemistry & Physics of Ministry of Education, Peking University, Beijing 100871, China.; Beijing NMR Center, School of Life Science, Peking University, Beijing 100871, China., Wang H; Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, National Biomedical Imaging Center, Key Laboratory of Polymer Chemistry & Physics of Ministry of Education, Peking University, Beijing 100871, China., Granick S; Department of Chemistry, Ulsan National Institute of Science and Technology, Ulsan 44919, South Korea.; Center for Soft and Living Matter, Institute for Basic Science, Ulsan 44919, South Korea.; Department of Polymer Science and Engineering, University of Massachusetts, Amherst, MA 01003.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Jan 16; Vol. 121 (3), pp. e2314797121. Date of Electronic Publication: 2024 Jan 09.
DOI:	10.1073/pnas.2314797121
Abstrakt:	Assessing the ergodicity of graphene liquid cell electron microscope measurements, we report that loop states of circular DNA interconvert reversibly and that loop numbers follow the Boltzmann distribution expected for this molecule in bulk solution, provided that the electron dose is low (80-keV electron energy and electron dose rate 1-20 e - Å -2 s -1 ). This imaging technique appears to act as a "slow motion" camera that reveals equilibrated distributions by imaging the time average of a few molecules without the need to image a spatial ensemble. Competing Interests: Competing interests statement:The authors declare no competing interest.
Databáze:	MEDLINE
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