Recombinant Pneumolysin of Pneumococci Induces TLR4 Expression and Maturation of Dendritic Cells In Vitro.

Autor: Akhmatova NK; I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia., Vorobyev DS; I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia., Petukhova ES; I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia., Semenova IB; I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia., Yakovleva IV; I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia., Gavrilova NF; I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia., Zaitsev AE; I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia., Akhmatova EA; I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia., Volokh YV; I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia., Leonova AY; I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia., Poddubikov AV; I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia., Kurbatova EA; I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia. kurbatova6162@yandex.ru.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2023 Dec; Vol. 176 (2), pp. 191-193. Date of Electronic Publication: 2024 Jan 08.
DOI: 10.1007/s10517-024-05993-5
Abstrakt: Pneumolysin (Ply) is a target for the development of serotype-independent pneumococcal vaccines, an important condition for the efficacy of which is their ability to activate innate immunity with the subsequent formation of adaptive immunity. In this study, the ability of recombinant full-length Ply (rPly) of pneumococci to induce TLR expression and maturation of dendritic cells generated from mouse bone marrow was evaluated. It was shown that rPly in vitro increased the number of dendritic cells expressing Toll-like receptor 4 (TLR4) on the membrane. rPly caused maturation of dendritic cells generated from mouse bone marrow, which manifested in a decrease in the number of progenitor cells (CD34), an increase in the number of cells expressing the adhesion molecule CD38, costimulatory molecules CD80 and CD86, molecules of terminal differentiation of dendritic cells CD83, as well as molecules of antigenic presentation of the major histocompatibility complex class II.
(© 2024. Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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