Single-cell mapping of lipid metabolites using an infrared probe in human-derived model systems.
Autor: | Bai Y; Neuroscience Research Institute, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA. yrbai@ucsb.edu.; Photothermal Spectroscopy Corp., Santa Barbara, CA, USA. yrbai@ucsb.edu., Camargo CM; Neuroscience Research Institute, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA., Glasauer SMK; Neuroscience Research Institute, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA., Gifford R; Neuroscience Research Institute, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA., Tian X; Neuroscience Research Institute, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA., Longhini AP; Neuroscience Research Institute, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA., Kosik KS; Neuroscience Research Institute, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA. kosik@lifesci.ucsb.edu. |
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Jazyk: | angličtina |
Zdroj: | Nature communications [Nat Commun] 2024 Jan 08; Vol. 15 (1), pp. 350. Date of Electronic Publication: 2024 Jan 08. |
DOI: | 10.1038/s41467-023-44675-0 |
Abstrakt: | Understanding metabolic heterogeneity is the key to uncovering the underlying mechanisms of metabolic-related diseases. Current metabolic imaging studies suffer from limitations including low resolution and specificity, and the model systems utilized often lack human relevance. Here, we present a single-cell metabolic imaging platform to enable direct imaging of lipid metabolism with high specificity in various human-derived 2D and 3D culture systems. Through the incorporation of an azide-tagged infrared probe, selective detection of newly synthesized lipids in cells and tissue became possible, while simultaneous fluorescence imaging enabled cell-type identification in complex tissues. In proof-of-concept experiments, newly synthesized lipids were directly visualized in human-relevant model systems among different cell types, mutation status, differentiation stages, and over time. We identified upregulated lipid metabolism in progranulin-knockdown human induced pluripotent stem cells and in their differentiated microglia cells. Furthermore, we observed that neurons in brain organoids exhibited a significantly lower lipid metabolism compared to astrocytes. (© 2024. The Author(s).) |
Databáze: | MEDLINE |
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