Bacillus velezensis BE2 controls wheat and barley diseases by direct antagonism and induced systemic resistance.

Autor: Dutilloy E; Université de Reims Champagne Ardenne, INRAE, RIBP USC 1488, 51100, Reims, France., Arias AA; Microbial Processes and Interactions Laboratory, Terra Teaching and Research Center, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium., Richet N; Université de Reims Champagne Ardenne, Plateau Technique Mobile de Cytométrie Environnementale MOBICYTE, URCA/INERIS, UFR Sciences Exactes Et Naturelles, Reims, France., Guise JF; Université de Reims Champagne Ardenne, INRAE, RIBP USC 1488, 51100, Reims, France., Duban M; Université de Lille, Université de Liège, UMRT, 1158 BioEcoAgro, Institut Charles Viollette, 59000, Lille, France., Leclere V; Université de Lille, Université de Liège, UMRT, 1158 BioEcoAgro, Institut Charles Viollette, 59000, Lille, France., Selim S; AGHYLE UP 2018.C101, SFR Condorcet FR CNRS 3417, Institut Polytechnique UniLaSalle, 19 Rue Pierre Waguet, BP 30313, F-60026, Beauvais Cedex, France., Jacques P; Microbial Processes and Interactions Laboratory, Terra Teaching and Research Center, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium., Jacquard C; Université de Reims Champagne Ardenne, INRAE, RIBP USC 1488, 51100, Reims, France., Clément C; Université de Reims Champagne Ardenne, INRAE, RIBP USC 1488, 51100, Reims, France., Ait Barka E; Université de Reims Champagne Ardenne, INRAE, RIBP USC 1488, 51100, Reims, France., Esmaeel Q; Université de Reims Champagne Ardenne, INRAE, RIBP USC 1488, 51100, Reims, France. qassim.esmaeel@univ-reims.fr.
Jazyk: angličtina
Zdroj: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2024 Dec; Vol. 108 (1), pp. 64. Date of Electronic Publication: 2024 Jan 08.
DOI: 10.1007/s00253-023-12864-y
Abstrakt: Wheat and barley rank among the main crops cultivated on a global scale, providing the essential nutritional foundation for both humans and animals. Nevertheless, these crops are vulnerable to several fungal diseases, such as Septoria tritici blotch and net blotch, which significantly reduce yields by adversely affecting leaves and grain quality. To mitigate the effect of these diseases, chemical fungicides have proven to be genuinely effective; however, they impose a serious environmental burden. Currently, biocontrol agents have attracted attention as a sustainable alternative to fungicides, offering an eco-friendly option. The study aimed to assess the efficacy of Bacillus velezensis BE2 in reducing disease symptoms caused by Zymoseptoria tritici and Pyrenophora teres. This bacterium exhibited significant antagonistic effects in vitro by suppressing fungal development when pathogens and the beneficial strain were in direct confrontation. These findings were subsequently confirmed through microscopic analysis, which illustrated the strain's capacity to inhibit spore germination and mycelial growth in both pathogens. Additionally, the study analysed the cell-free supernatant of the bacterium using UPLC-MS (ultra-performance liquid chromatography-mass spectrometry). The results revealed that strain BE2 produces, among other metabolites, different families of cyclic lipopeptides that may be involved in biocontrol. Furthermore, the beneficial effects of strain BE2 in planta were assessed by quantifying the fungal DNA content directly at the leaf level after bacterization, using two different application methods (foliar and drenching). The results indicated that applying the beneficial bacterium at the root level significantly reduced pathogens pressure. Finally, gene expression analysis of different markers showed that BE2 application induced a priming effect within the first hours after infection. KEY POINTS: • BE2 managed Z. tritici and P. teres by direct antagonism and induced systemic resistance. • Strain BE2 produced seven metabolite families, including three cyclic lipopeptides. • Application of strain BE2 at the root level triggered plant defense mechanisms.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: