De Novo Green Fluorescent Protein Chromophore-Based Probes for Capturing Latent Fingerprints Using a Portable System.

Autor: Ruan N; The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Frontiers Science Research Base of Biomimetic Catalysis, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China., Qiu Q; The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Frontiers Science Research Base of Biomimetic Catalysis, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China., Wei X; The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Frontiers Science Research Base of Biomimetic Catalysis, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China., Liu J; The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Frontiers Science Research Base of Biomimetic Catalysis, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China., Wu L; The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Frontiers Science Research Base of Biomimetic Catalysis, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China.; Department of Chemistry, University of Bath, Bath BA2 7AY, U.K., Jia N; The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Frontiers Science Research Base of Biomimetic Catalysis, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China., Huang C; The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Frontiers Science Research Base of Biomimetic Catalysis, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China., James TD; The Education Ministry Key Laboratory of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Frontiers Science Research Base of Biomimetic Catalysis, Department of Chemistry, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, China.; Department of Chemistry, University of Bath, Bath BA2 7AY, U.K.; School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.
Jazyk: angličtina
Zdroj: Journal of the American Chemical Society [J Am Chem Soc] 2024 Jan 24; Vol. 146 (3), pp. 2072-2079. Date of Electronic Publication: 2024 Jan 08.
DOI: 10.1021/jacs.3c11277
Abstrakt: Rapid visualization of latent fingerprints, preferably at their point of origin, is essential for effective crime scene evaluation. Here, we present a new class of green fluorescent protein chromophore-based fluorescent dyes (LFP-Yellow and LFP-Red) that can be used for real-time visualization of LFPs within 10 s. Compared with traditional chemical reagents for LFPs, these fluorescent dyes are completely water-soluble, exhibit low cytotoxicity, and are harmless to users. Level 1-3 details of the LFPs could be clearly revealed through "off-on" fluorescence signal readout. Additionally, the fluorescent dyes were constructed based on an imidazolinone core and so do not contain pyridine groups or metal ions, which ensures that the DNA is not contaminated during extraction and identification after the LFPs are treated with the dyes. Combined with our as-developed portable system for capturing LFPs, LFP-Yellow and LFP-Red enabled the rapid capture of LFPs. Therefore, these green fluorescent protein chromophore-based probes provide an approach for the rapid identification of individuals who were present at a crime scene.
Databáze: MEDLINE
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